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AB276059

重组Anti-CPLX2抗体[021] - BSA and Azide free

Anti-CPLX2 antibody [021] - BSA and Azide free

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Rabbit Recombinant Monoclonal CPLX2 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus.

查看别名

Complexin-2, Complexin II, Synaphin-1, CPX II, CPLX2

2 Images
Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)

Intracellular flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059, followed byFITC-conjugated secondary antibody (black line) compared with a negative control (grey line).The cells were treated according to manufacturer's manual. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)
  • ICC

Unknown

Immunocytochemistry - Anti-CPLX2 antibody [021] - BSA and Azide free (AB276059)

Immunofluorescent analysis of 4% PFA fixed, 0.1% Triton X-100 in PBS permeabilzed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling CPLX2 with ab276059 at 1/60 dilution, 4°C overnight, followed by Goat Anti-rabbit IgG Alexa Fluor 488-conjugated secondary antibody (green). Cells were blocked with 10% serum prior incubation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

021

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

Flow Cyt (Intra), ICC

applications

免疫原

Recombinant Fragment Protein within Human CPLX2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PUV4

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搭配使用产品

Sample Prep & Detection Kits

AB102884

FITC Conjugation Kit - Lightning-Link®

Immunocytochemistry - FITC Conjugation Kit - Lightning-Link® (AB102884)

  • 5
  • 3
Sample Prep & Detection Kits

AB270709

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

  • 0
  • 0
Primary Antibodies

AB3340

Anti-SNAP23 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (AB3340)

  • 4
  • 7
Primary Antibodies

AB290726

Anti-VAMP4 antibody [EPR25066-77]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP4 antibody [EPR25066-77] (AB290726)

  • 0
  • 0
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 39
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

CPLX2 also known as Complexin-2 is a synaptic protein involved in neurotransmitter release mechanism. It has a molecular mass of approximately 15 kDa. CPLX2 is highly expressed in the brain and is particularly abundant in synaptic vesicles of neuronal cells. It interacts with SNARE proteins which play a significant role in the vesicle fusion process. CPLX2 acts as a regulator of neurotransmitter release influencing the balance between excitation and inhibition in synaptic transmission.
Biological function summary

CPLX2 functions by interacting with other synaptic proteins to facilitate neurotransmitter release at synapses. It forms a complex with SNARE proteins which are essential for synaptic vesicle fusion with the presynaptic membrane. This interaction ensures precise timing of neurotransmitter release which is critical for neural communication. CPLX2’s role in synaptic function highlights its importance in maintaining the efficiency of synaptic signaling.

Pathways

The role of CPLX2 is integral in synaptic vesicle cycle and neurotransmission pathways. It closely interacts with SNARE proteins such as Syntaxin and Synaptobrevin in facilitating vesicle docking and fusion. These interactions are essential for the neurotransmitter release process which is part of the larger synaptic signaling pathway. CPLX2 modulates synaptic plasticity and exocytosis linking it to the regulation of synaptic strength and neural network activity.

Alterations in CPLX2 expression or function have been associated with neurological conditions like epilepsy and schizophrenia. Abnormal CPLX2 activity may lead to disrupted synaptic signaling contributing to the pathophysiology of these disorders. Additionally its connection with SNARE proteins such as SNAP-25 serves as a potential point of intersection where synaptic dysfunction can contribute to disease development. Understanding CPLX2’s influence on neurotransmission provides insights into therapeutic targets for synaptic disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Negatively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Also involved in mast cell exocytosis (By similarity).
See full target information CPLX2
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