Anti-CPI-17 (phospho T38)抗体(ab52174)
Key features and details
- Rabbit polyclonal to CPI-17 (phospho T38)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CPI-17 (phospho T38)抗体
参阅全部 CPI-17 一抗 -
描述
兔多克隆抗体to CPI-17 (phospho T38) -
宿主
Rabbit -
特异性
ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CPI-17 aa 1-100 (phospho T38).
Database link: Q96A00 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| WB | (2) |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
靶标
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功能
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. -
组织特异性
Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. -
序列相似性
Belongs to the PP1 inhibitor family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 94274 Human
- Entrez Gene: 68458 Mouse
- Omim: 608153 Human
- SwissProt: Q96A00 Human
- SwissProt: Q91VC7 Mouse
- Unigene: 631569 Human
- Unigene: 2343 Mouse
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别名
- 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
- 17-KDa protein antibody
- 17kDa PKC potentiated inhibitory protein of PP1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (ab52174)
ab52174 at 1/50 dilution staining CPI-17 in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.
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Western blot - Anti-CPI-17 (phospho T38) antibody (ab52174)Western blot analysis of extracts from RAW264.7 cells, using CPI-17 (Phospho-Thr38) (ab52174)Antibody. The lane on the right is treated with the synthesized peptide.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab52174 被引用在 5 文献中.
- Lin Z et al. Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction. Evid Based Complement Alternat Med 2020:9189457 (2020). PubMed: 32104199
- Ge J et al. S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cß/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway. Exp Ther Med 16:103-112 (2018). PubMed: 29896230
- Chen J et al. Roles and mechanisms of TRPC3 and the PLC?/PKC/CPI-17 signaling pathway in regulating parturition. Mol Med Rep 17:898-910 (2018). PubMed: 29115500
- Yin Y et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
- Kang S et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . PubMed: 24449418