Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-Coxsackie Adenovirus Receptor/hCAR抗体[EPR23305-44] - BSA and Azide free (ab272939)

Submit a review Submit a question
Immunocytochemistry/ Immunofluorescence - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
  • Immunoprecipitation - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
  • Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
  • Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23305-44] to Coxsackie Adenovirus Receptor/hCAR - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Coxsackie Adenovirus Receptor/hCAR抗体[EPR23305-44] - BSA and Azide free
    参阅全部 Coxsackie Adenovirus Receptor/hCAR 一抗

  • 描述

    兔单克隆抗体[EPR23305-44] to Coxsackie Adenovirus Receptor/hCAR - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, Flow Cyt, IPmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: LoVo, HepG2, HaCaT, BxPC-3, Human brain and Human liver, Human heart lysates. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells. IP: HepG2 cells.

  • 常规说明

    ab272939 is the carrier-free version of ab272711.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272939于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1/JAML located in the plasma membrane of PMN.

  • 组织特异性

    Expressed in pancreas, brain, heart, small intestine, testis, prostate and at a lower level in liver and lung. Isoform 5 is ubiquitously expressed. Isoform 3 is expressed in heart, lung and pancreas. In skeletal muscle, isoform 1 is found at the neuromuscular junction and isoform 2 is found in blood vessels. In cardiac muscle, isoform 1 and isoform 2 are found at intercalated disks. In heart expressed in subendothelial layers of the vessel wall but not in the luminal endothelial surface. Expression is elevated in hearts with dilated cardiomyopathy.

  • 序列相似性

    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • 结构域

    The Ig-like C2-type 1 domain probably mediates homodimerization and interaction with JAML.
    The PDZ-binding motif mediates interaction with MPDZ and BAIAP1.

  • 翻译后修饰

    N-glycosylated.
    Palmitoylated on Cys-259 and/or Cys-260; required for proper localization to the plasma membrane.

  • 细胞定位

    Secreted and Cell membrane. Cell junction > tight junction. Cell junction > adherens junction. Basolateral cell membrane. In epithelial cells localizes to the apical junction complex composced of tight and adherens junctions. In airway epithelial cells localized to basolateral membrane but not to apical surface.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 46 kD coxsackievirus and adenovirus receptor CAR protein antibody
      • 46 kD coxsackievirus and adenovirus receptor protein antibody
      • CAR antibody
      • CAR4/6 antibody
      • Coxsackie virus and adenovirus receptor antibody
      • Coxsackie virus B receptor antibody
      • Coxsackievirus and adenovirus receptor antibody
      • Coxsackievirus B adenovirus receptor antibody
      • Coxsackievirus B-adenovirus receptor antibody
      • CVB3 binding protein antibody
      • CVB3-binding protein antibody
      • Cxadr antibody
      • CXADR Ig like cell adhesion molecule antibody
      • CXAR_HUMAN antibody
      • hCAR antibody
      • HCVADR antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
      Immunocytochemistry/ Immunofluorescence - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Coxsackie Adenovirus Receptor/hCAR with ab272711 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in HepG2 cell line. Negative control: K562 (PMID: 30450519) ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272711).

    • Immunoprecipitation - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
      Immunoprecipitation - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)

      Coxsackie Adenovirus Receptor/hCAR was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab272711 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272711 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug

      Lane 2: ab272711 IP in HepG2 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272711 in HepG2 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 102 seconds

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272711).

    • Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
      Flow Cytometry - Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)

      Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Coxsackie Adenovirus Receptor/hCAR with ab272711 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562 (PMID: 30450519) Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272711).

    • Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)
      Anti-Coxsackie Adenovirus Receptor/hCAR antibody [EPR23305-44] - BSA and Azide free (ab272939)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272939？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272939 尚未被引用在任何文献中。

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab272939.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com