重组Anti-COX6B1抗体[EPR7647] (ab131277)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.cn/ps/products/131/ab131277/Images/ab131277-145662-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7647] to COX6B1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-COX6B1抗体[EPR7647]
参阅全部 COX6B1 一抗 -
描述
兔单克隆抗体[EPR7647] to COX6B1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HL60, HeLa, HepG2 and Caco 2 cell lysates; HepG2 cells;. Human colon tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 2.49 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7647 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131277于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
|
|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF |
1/50 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
靶标
-
功能
Connects the two COX monomers into the physiological dimeric form. -
疾病相关
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
序列相似性
Belongs to the cytochrome c oxidase subunit 6B family. -
细胞定位
Mitochondrion intermembrane space. - Information by UniProt
-
数据库链接
- Entrez Gene: 1340 Human
- Omim: 124089 Human
- SwissProt: P14854 Human
- Unigene: 431668 Human
-
别名
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX6B1 antibody [EPR7647] (ab131277)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling COX6B1 with ab131277 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.cn/ps/products/131/ab131277/Images/ab131277-145663-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-western-blot.jpg)
Western blot - Anti-COX6B1 antibody [EPR7647] (ab131277)All lanes : Anti-COX6B1 antibody [EPR7647] (ab131277) at 1/1000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 10 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.cn/ps/products/131/ab131277/Images/ab131277-145661-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [EPR7647] (ab131277)Immunofluorescent analysis of HepG2 cells labelling COX6B1 with ab131277 at 1/50 dilution.
-
![OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.cn/ps/products/131/ab131277/Images/ab131277-145660-anti-cytochrome-c-oxidase-subunit-vib-antibody-epr7647.jpg)
OI-RD Scanning - Anti-COX6B1 antibody [EPR7647] (ab131277) -
![Anti-COX6B1 antibody [EPR7647] (ab131277)](https://www.abcam.cn/ps/products/131/ab131277/Images/ab131277-5-benefits-of-recombinant-antibodies.png)
Anti-COX6B1 antibody [EPR7647] (ab131277)
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab131277 被引用在 1 文献中.
- Fan W et al. Glioma cells are resistant to inflammation-induced alterations of mitochondrial dynamics. Int J Oncol 57:1293-1306 (2020). PubMed: 33174046