AB110265

Anti-COX6A1抗体[14A3AD2BH4]

Name: COX6A1抗体[14A3AD2BH4] (ab110265)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110265
Price: 4519 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-COX6A1 antibody [14A3AD2BH4]

(1 Review)

(4 Publications)

Mouse Monoclonal COX6A1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat, Mouse samples. Cited in 4 publications.

查看别名

Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit SSG

2 Images

Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

Flow Cyt

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Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

Overlay histogram showing HepG2 cells stained with ab110265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab110265, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

Extra bands in the mouse sample (lane 3) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.

All lanes:

Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (ab110265) at 5 µg/mL

Lane 1:

Human Heart Lysate

Lane 2:

Rat Heart Lysate

Lane 3:

Mouse Heart Lysate

Lane 4:

HepG2 cell lysate

Predicted band size: 12 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

14A3AD2BH4

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

ab110265 recognises the Liver isoform in human, rat, mouse.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "5 µg/mL", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化说明

ab110265 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

COX6A1 also known as Cytochrome c oxidase subunit 6A1 plays an important role in the electron transport chain by being part of the cytochrome c oxidase (complex IV) in mitochondria. This subunit has a mass of approximately 14 kDa. COX6A1 expresses in various human tissues but shows higher expression in tissues with significant energy demands such as the heart and skeletal muscle. This evidence suggests its critical involvement in cellular energy production.

Biological function summary

COX6A1 contributes to the formation of the cytochrome c oxidase complex which is the terminal enzyme in the mitochondrial respiratory chain. This complex facilitates the transfer of electrons from cytochrome c to oxygen leading to ATP production through oxidative phosphorylation. COX6A1 is an integral component of this complex which exists as part of the inner mitochondrial membrane. It interacts with other subunits to ensure efficient electron transfer and energy conversion.

Pathways

COX6A1 heavily influences the mitochondrial respiratory pathway particularly in oxidative phosphorylation. This pathway includes important components like NADH: ubiquinone oxidoreductase and cytochrome c. COX6A1 bridges interactions within the complex IV with proteins such as COX1 enabling proper electron transport and proton pumping necessary for ATP synthesis. Dysfunction in these interactions may affect overall cellular respiration efficiency.

Mutations and dysregulation of COX6A1 relate to various mitochondrial disorders and neurodegenerative conditions such as Leigh syndrome. Its interactions with other mitochondrial proteins like COX2 and COX3 are vital for maintaining mitochondrial function. Deficiencies in COX6A1 can disrupt proper electron transport leading to decreased ATP production and contributing to pathology in energy-demanding tissues like the brain and muscles.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules unsing 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX6A1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadq1575 PubMed40184463

2025

Atf3 controls transitioning in female mitochondrial cardiomyopathy as identified by spatial and single-cell transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Tasneem Qaqorh,Yusuke Takahashi,Kohei Sameshima,Kentaro Otani,Issei Yazawa,Yuya Nishida,Kohei Tonai,Yoshitaka Fujihara,Mizuki Honda,Shinya Oki,Yasuyuki Ohkawa,David R Thorburn,Ann E Frazier,Atsuhito Takeda,Yoshihiko Ikeda,Heima Sakaguchi,Takuya Watanabe,Norihide Fukushima,Yasumasa Tsukamoto,Naomasa Makita,Osamu Yamaguchi,Kei Murayama,Akira Ohtake,Yasushi Okazaki,Takanari Kimura,Hisakazu Kato,Hijiri Inoue,Ken Matsuoka,Seiji Takashima,Yasunori Shintani

Oxidative medicine and cellular longevity 2023:4202861 PubMed36647431

2023

Psoraleae Fructus Ethanol Extract Induced Hepatotoxicity via Impaired Lipid Metabolism Caused by Disruption of Fatty Acid -Oxidation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaojuan Guo,Yuanyuan Shi,Bingqian Jiang,Xiyi Peng,Lin Zhang,Can Tu,Ting Wang

American journal of human genetics 95:294-300 PubMed25152455

2014

A mutation of COX6A1 causes a recessive axonal or mixed form of Charcot-Marie-Tooth disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gen Tamiya,Satoshi Makino,Makiko Hayashi,Akiko Abe,Chikahiko Numakura,Masao Ueki,Atsushi Tanaka,Chizuru Ito,Kiyotaka Toshimori,Nobuhiro Ogawa,Tomoya Terashima,Hiroshi Maegawa,Daijiro Yanagisawa,Ikuo Tooyama,Masayoshi Tada,Osamu Onodera,Kiyoshi Hayasaka

Mechanisms of ageing and development 129:558-61 PubMed18538373

2008

Age-related decrease in expression of mitochondrial DNA encoded subunits of cytochrome c oxidase in Drosophila melanogaster.

Applications

Unspecified application

Species

Unspecified reactive species

Rajindar S Sohal,Dikran Toroser,Catherine Brégère,Robin J Mockett,William C Orr

View all publications

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Anti-COX6A1抗体[14A3AD2BH4]

Anti-COX6A1 antibody [14A3AD2BH4]

Flow Cytometry - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

Western blot - Anti-COX6A1 antibody [14A3AD2BH4] (AB110265)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原

特异性