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重组RabMAb

重组Anti-COX5B抗体[EPR14439(B)] (ab180136)

Reviews (1) Submit a question References (6)
Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Flow Cytometry (Intracellular) - Anti-COX5B antibody [EPR14439(B)] (ab180136)
  • Anti-COX5B antibody [EPR14439(B)] (ab180136)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14439(B)] to COX5B
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-COX5B抗体[EPR14439(B)]
    参阅全部 COX5B 一抗

  • 描述

    兔单克隆抗体[EPR14439(B)] to COX5B

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human COX5B aa 1-100. The exact sequence is proprietary.
    Database link: P10606

  • 阳性对照

    • Human fetal heart lysate; HeLa and HepG2 cells; Human kidney tissue; U87-MG, HepG2 and HeLa cell lysates.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180136于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
1/10000 - 1/50000. Predicted molecular weight: 14 kDa.
ICC/IF
1/1000.
IP
1/70.
说明
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Predicted molecular weight: 14 kDa.
ICC/IF
1/1000.
IP
1/70.

靶标

图片

  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)This image is courtesy of an Abreview submitted by Divya Nagarajan
    All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution

    Lane 1 : IMR32- human neuroblastoma whole cell lysate
    Lane 2 : 9464D- murine Neuroblastoma whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Anti-rabbit IgG, HRP-linked Antibody at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 14,50 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    4-12% gel reducing denaturing conditions,  blocked using 5% milk for 1 hour at 23°. 
    145kDa - Vinculin (loading control). Samples were incubated with the primary antiody at 10000 dilution for 20 hours at 4° in 1X TBS,0.1% Tween-20,5% BSA.
    14kDa band specific, 50kDa band non specific

    See Abreview

  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    All lanes : Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/50000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunocytochemistry/ Immunofluorescence - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunofluorescence analysis of HepG2 cells labeling COX5B using ab180136 at 1/1000 dilution. A Goat anti rabbit IgG (Dylight 555) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling COX5B using ab180136 at 1/500 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Immunoprecipitation - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Immunoprecipitation analysis of Human fetal heart lysate labeling COX5B using ab180136 at 1/70 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Western blot - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Anti-COX5B antibody [EPR14439(B)] (ab180136) at 1/10000 dilution + U87-MG cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Flow Cytometry (Intracellular) - Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Flow Cytometry (Intracellular) - Anti-COX5B antibody [EPR14439(B)] (ab180136)

    Intracellular Flow Cytometry analysis of HeLa cells labeling COX5B using ab180136 at 1/10 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG. 

     

     

  • Anti-COX5B antibody [EPR14439(B)] (ab180136)
    Anti-COX5B antibody [EPR14439(B)] (ab180136)

文献 (6)

发表研究结果有使用 ab180136？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab180136 被引用在 6 文献中.

  • Chu YD  et al. COX5B-Mediated Bioenergetic Alterations Modulate Cell Growth and Anticancer Drug Susceptibility by Orchestrating Claudin-2 Expression in Colorectal Cancers. Biomedicines 10:N/A (2021). PubMed: 35052740
  • Chu YD  et al. COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma. Cancers (Basel) 12:N/A (2020). PubMed: 32580279
  • Li Y  et al. Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins. Oxid Med Cell Longev 2020:5408452 (2020). PubMed: 32587661
  • Stein J  et al. Systematic expression analysis of the mitochondrial respiratory chain protein subunits identifies COX5B as a prognostic marker in clear cell renal cell carcinoma. Int J Urol 26:910-916 (2019). PubMed: 31280487
  • Wang T  et al. Pyridine nucleotide-disulphide oxidoreductase domain 2 (PYROXD2): Role in mitochondrial function. Mitochondrion 47:114-124 (2019). PubMed: 31170524
  • Mot AI  et al. Circumventing the Crabtree Effect: A method to induce lactate consumption and increase oxidative phosphorylation in cell culture. Int J Biochem Cell Biol 79:128-138 (2016). PubMed: 27590850

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Western blot abreview for Anti-COX5B antibody [EPR14439(B)]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
35 µg
Specification
neuroblastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Divya Nagarajan

Verified customer

提交于 Feb 23 2022

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