AB110272

Anti-COX4，mitochondrial抗体[1A12A12]

Name: COX4, mitochondrial抗体[1A12A12] (ab110272)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110272
Price: 4281 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-COX4, mitochondrial antibody [1A12A12]

(1 Review)

(28 Publications)

Mouse Monoclonal COX4, mitochondrial antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 28 publications.

查看别名

YGL187C, G1362, COX4, Cytochrome c oxidase polypeptide IV

1 Images

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Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (AB110272)

All lanes:

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272) at 1 µg/mL

All lanes:

Mitochondria from yeast membrane extract at 20 µg

Predicted band size: 17 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1A12A12

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Saccharomyces cerevisiae

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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产品详情

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯度

IgG fraction

纯化说明

Near homogeneity as judged by SDS-PAGE. ab110272 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

COX4 also known as cytochrome c oxidase subunit IV is a component of the mitochondrial respiratory chain. It has an approximate molecular weight of 17 kDa. COX4 is expressed in high levels in tissues with abundant energy requirements such as heart muscle and brain. This protein is part of the cytochrome c oxidase (complex IV) the last enzyme in the mitochondrial electron transport chain which plays an important role in cellular respiration.

Biological function summary

The cytochrome c oxidase complex facilitates the transfer of electrons from cytochrome c to oxygen ultimately generating water. This process contributes to the proton gradient across the mitochondrial membrane necessary for ATP synthesis. COX4 serves as a regulatory subunit that can influence the efficiency of the electron transfer process. This complex is vital for maintaining the balance of energy production in cells and has a role in adaptive responses to varying energy demands.

Pathways

COX4 functions in the oxidative phosphorylation pathway which is critical for ATP production in eukaryotic cells. This pathway includes several protein complexes with COX4 being a part of the terminal complex IV. It interacts with other proteins such as cytochrome c and ATP synthase linking electron transport to ATP production. In turn this pathway supports various biological functions and maintains cellular energy homeostasis.

Dysfunctional COX4 is associated with mitochondrial diseases that often lead to compromised energy production. Conditions such as mitochondrial myopathy and cytochrome c oxidase deficiency have been linked to functional anomalies in COX4. These disorders can affect muscles and neural tissues leading to symptoms such as muscle weakness and neurological impairments. COX4 acts with proteins like cytochrome c and other subunits of the cytochrome c oxidase complex in these disease mechanisms influencing the severity and progression of these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of COX2 and heme A of COX1 to the active site in COX1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX4

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5314 PubMed40595511

2025

A constricted mitochondrial morphology formed during respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Manish K Singh,Laetitia Cavellini,Maria Angeles Morcillo-Parra,Christina Kunz,Mickaël Lelek,Perrine Bomme,Aurélia Barascu,Cynthia Alsayyah,Maria Teresa Teixeira,Naïma Belgareh-Touzé,Adeline Mallet,Lea Dietrich,Christophe Zimmer,Mickael M Cohen

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2407705 PubMed39447130

2024

Unfractionated Heparin Enhances Sepsis Prognosis Through Inhibiting Drp1-Mediated Mitochondrial Quality Imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixue Liu,He Huang,Dongyao Hou,Shuai Hao,Qiao Guo,Haitang Liao,Rui Song,Yu Tian,Qian Chen,Zhenchun Luo,Daqing Ma,Liangming Liu,Chenyang Duan

Methods in enzymology 700:77-104 PubMed38971613

2024

Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Israel Juarez-Contreras,Itay Budin

The Journal of biological chemistry 300:105496 PubMed38013088

2023

Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Itay Budin

The Journal of clinical investigation 132: PubMed35426376

2022

Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Yu Zou,Lin Guo,Bei Zhang,Si Chen,Xin-Rong Wu,Xian-Dong Liu,Xin-Yu Xu,Bin-Yin Li,Shengdi Chen,Nan-Jie Xu,Suya Sun

Clinical science (London, England : 1979) 136:45-60 PubMed34918039

2021

Inhibition of HDAC3 protects against kidney cold storage/transplantation injury and allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xiang,Guie Dong,Jiefu Zhu,Gang Zhang,Zheng Dong

Journal of biochemical and molecular toxicology 36:e22939 PubMed34719845

2021

Genistein suppresses ox-LDL-elicited oxidative stress and senescence in HUVECs through the SIRT1-p66shc-Foxo3a pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Zhang,Xuefen Pang,Haixia Yu,Hui Zhou

PLoS genetics 17:e1009808 PubMed34665800

2021

Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Corbi,Angelika Amon

View all publications

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Abcam Product Promise

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Anti-COX4，mitochondrial抗体[1A12A12]

Anti-COX4, mitochondrial antibody [1A12A12]

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (AB110272)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原