Anti-COX20抗体- C-terminal (ab224570)
Key features and details
- Rabbit polyclonal to COX20 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-COX20抗体- C-terminal -
描述
兔多克隆抗体to COX20 - C-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human COX20 aa 50 to the C-terminus (C terminal).
Database link: Q5RI15 -
阳性对照
- IHC-P: Human kidney tissue. ICC/IF: U-2 OS cells. WB: A-431 and HEK 293 cell lines.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa.
|
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 13 kDa. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
COX20 is a multi-pass membrane protein. It belongs to the COX20 family. The exact function of COX20 remains unknown. -
细胞定位
Membrane; Multi-pass membrane protein. -
数据库链接
- Entrez Gene: 116228 Human
- SwissProt: Q5RI15 Human
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别名
- COX20 antibody
- COX20 cytochrome c oxidase assembly factor antibody
- Cytochrome c oxidase protein 20 homolog antibody
see all
图片
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Western blot - Anti-COX20 antibody - C-terminal (ab224570)All lanes : Anti-COX20 antibody - C-terminal (ab224570) at 0.4 µg/ml
Lane 1 : A-431 cell line
Lane 2 : HEK 293 cell line
Predicted band size: 13 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-COX20 antibody - C-terminal (ab224570)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for COX20 (green) using ab224570 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX20 antibody - C-terminal (ab224570)Paraffin-embedded human kidney tissue stained for COX20 with ab224570 at 1/50 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab224570 被引用在 1 文献中.
- Li P et al. Compound Heterozygous COX20 Variants Impair the Function of Mitochondrial Complex IV to Cause a Syndrome Involving Ophthalmoplegia and Visual Failure. Front Neurol 13:873943 (2022). PubMed: 35651336