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重组RabMAb

重组Anti-COX2 / Cyclooxygenase 2抗体[SP21] (ab16701)

Reviews (1)Q&A (4)References (7)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
    • Suitable for: IHC-P
    • Reacts with: Human

    概述

    • 产品名称

      Anti-COX2 / Cyclooxygenase 2抗体[SP21]
      参阅全部 COX2 / Cyclooxygenase 2 一抗

    • 描述

      兔单克隆抗体[SP21] to COX2 / Cyclooxygenase 2

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details
      不适用于: WB

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat

    • 免疫原

      Synthetic peptide within Rat COX2/ Cyclooxygenase 2 (C terminal). The exact sequence is proprietary.

    • 阳性对照

      • IHC-P: Human colon carcinoma tissue.

    • 常规说明

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab16701于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    说明
    IHC-P
    1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    应用说明
    Is unsuitable for WB.

    靶标

    • 功能

      Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

    • 通路

      Lipid metabolism; prostaglandin biosynthesis.

    • 序列相似性

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.

    • 翻译后修饰

      S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561.

    • 细胞定位

      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • COX 2 antibody
      • COX-2 antibody
      • COX2 antibody
      • Cyclooxygenase 2 antibody
      • Cyclooxygenase 2b antibody
      • Cyclooxygenase antibody
      • Cyclooxygenase-2 antibody
      • Cyclooxygenase2 antibody
      • EC 1.14.99.1 antibody
      • fj02a10 antibody
      • Glucocorticoid-regulated inflammatory cyclooxygenase antibody
      • Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody
      • GRIPGHS antibody
      • hCox 2 antibody
      • Macrophage activation-associated marker protein P71/73 antibody
      • OTTHUMP00000033524 antibody
      • PES-2 antibody
      • PGG/HS antibody
      • PGH synthase 2 antibody
      • PGH2_HUMAN antibody
      • PGHS 2 antibody
      • PGHS-2 antibody
      • PGHS2 antibody
      • PHS 2 antibody
      • PHS II antibody
      • PHS2 antibody
      • Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin endoperoxide synthase 2 antibody
      • Prostaglandin G/H synthase 2 antibody
      • Prostaglandin G/H synthase 2 precursor antibody
      • Prostaglandin G/H synthase and cyclooxygenase antibody
      • Prostaglandin G/H synthase antibody
      • Prostaglandin H2 synthase 2 antibody
      • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 2 antibody
      • PTGS2 antibody
      • ptgs2a antibody
      • TIS10 antibody
      • TIS10 protein antibody
      • unp1239 antibody
      • wu:fj02a10 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)

      Immunohistochemical analysis of paraffin-embedded Human colon carcinoma labeling COX2/Cyclooxygenase 2 with ab16701 at 1/100 (6 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16701 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.

    文献 (7)

    发表研究结果有使用 ab16701？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab16701 被引用在 7 文献中.

    • Madhavan MK  et al. Aberrant uterine folding in mice disrupts implantation chamber formation and alignment of embryo-uterine axes. Development 149:N/A (2022). PubMed: 35575097
    • Szlendak M  et al. Expression of cyclooxygenase-2 and mucin 1 in colorectal cancer. Mol Clin Oncol 13:52 (2020). PubMed: 32874582
    • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
    • Peng YJ  et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173
    • Szylberg L  et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). PubMed: 26925134
    • Fujita M  et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). PubMed: 21324923
    • Lu TC  et al. Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin. Evid Based Complement Alternat Med : (2009). PubMed: 19933324

    客户评价及客户问答

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX2 / Cyclooxygenase 2 antibody [SP21]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver, GIT, kidney, ovary and oviduct)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
    Permeabilization
    Yes - 0.2% Triton in PBSx1
    Specification
    liver, GIT, kidney, ovary and oviduct
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Einav Bangiev

    Verified customer

    提交于 Jun 13 2023

    Question

    I was wondering if you can provide us an information about concentration of that antibody in ug/ml (or in similar units) OR give us the information how much antibodies are in those 500 microliters in micrograms, milligrams or grams.

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    Answer

    Unfortunately it is not possible to determine the concentration since tissue culture supernatant will contain many other host proteins besides the antibody of interest as it is unpurified. As a rough guide tissue culture supernatant contains 1 mg/ml total antibodies and 0.05 mg/ml specific antibodies.

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    Question

    BATCH NUMBER 237901 ORDER NUMBER 196732 DESCRIPTION OF THE PROBLEM There are strong bands at the wrong band sizes (see attached file). SAMPLE Rat liver hepatoma, H4IIE-cells treated with TNF-alpha PRIMARY ANTIBODY abcam, ab16701-500 Rabbit monoclonal [SP21] to COX2/ Cyclooxygenase 2 diluted 1/200 in 1% milk powder in TBS-Tween incubation time: 2.5 hours at room temperature agitating wash step: 3 x 5 min with TBS-Tween DETECTION METHOD ECL, substrate and peroxide buffer from tebu-bio : Chemiluminescent Sensitive HRP Substrate Kit (SuperDetect);2-Component Chemiluminescent Substrate for the Detection of Horseradish Peroxidase. Detection with Lumi-Imager from Roche (Lumi-Analyst Software) or X-ray films. ANTIBODY STORAGE CONDITIONS +4°C in storage buffer (data sheet) SAMPLE PREPARATION H4IIE-cells were treated with different concentrations of TNF-alpha for 6 hours. Incubation scheme: 1 000 000 cells per 60 mm petri dish were sowed in DMEM low glucose containing 10% BSA and 1% P/S and left to adhere for 24 hours at 37 °C. Then the culture medium was evacuated and the cells were washed with 0,9 % sodium chloride. Then The cells were incubated with 10, 30, 50 or 70 ng/ml TNF-alpha (dissolved in culture medium) for 6 hours. After 6 hours the incubation medium was evacuated and the cells were washed three times with 0,9 % sodium chloride. Then the cells were incubated with culture medium for another 24 hours. After 24 hours the medium was evacuated and cells were washed with 0,9 % sodium chloride. Cell lysis has been carried out by harvesting the cells with 70 µl RIPA-Lysis-buffer per 60 mm dish (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 1% Triton X-100, 1 % sodium deoxycholate, 0,1 % SDS, 50 µl Protease Inhibitor Cocktail from sigma). Cell suspension was then incubated for 15 min on ice with gentle agitation and centrifugated for 15 min with 14 000 x g at 4 °C. Supernatant was evacuated and stored at -80°C until protein determination by Bradford Assay.Samples were diluted with RIPA-buffer to 4 µg/µl total protein. From this solution 12 µl were diluted with 6 µl Laemmli buffer (3x) and heated up to 95 °C for 5-6 min. The samples were pulsed and loaded onto the gel. AMOUNT OF PROTEIN LOADED 48 µg total protein per lane, protein determined by Bradford Assay ELECTROPHORESIS/GEL CONDITIONS stacking: 4 % SDS-polyacrylamidgel separating/resolving: 10% SDS-polyacrylamidgel buffer: 2 M glycine, 250 mM Tris, 1 % SDS run: 70 V for 15 min, 100 V for 1 h 40 min TRANSFER AND BLOCKING CONDITIONS discontinuous buffer system: anode buffer I : 300 mM Tris, 10 % methanol, pH 10.4 anode buffer II: 25 mM Tris, 10 % methanol, pH 10.4 cathode buffer: 40 mM glycine, 25 mM Tris, 10 % methanol, SDS, pH 9.4 time period: 1 h 25 min at 90 mA per membrane PVDF membrane (Millipore) blocking conditions: 1 h at room temperature with 5 % milk powder in TBS wash step after blocking: 3 x 5 min with TBS-Tween SECONDARY ANTIBODY [another company]./ goat anti rabbit HRP-linked diluted 1/1000 in 1 % milk powder in TBS-Tween incubation time: 1.5 hours at room temperature agitating wash step: 3 x 5 min with TBS-Tween HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? washing steps reduced, electrophoresis run extended, blotting time extended, new dilution of frozen samples, ADDITIONAL NOTES The attached file shows the last western blot. Loaded samples: H4IIE-Lysate (as described above) from left to right: 10, 30, 50, 70 ng/ml TNF-alpha incubation (6h); 48 µg total protein per lane. Two different times of exposition (30 sec and 300 sec) . Detected with chemiluminescence substrate from tebu-bio and Lumi-Imager (Roche).

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    Answer

    I'm very sorry to hear you are experiencing problems with ab16701. The protocol you have used is very good and should work well; in light of your problems it is clear that the antibody did not work well in western blotting. I would like to offer you a refund or credit note on your order, or another anti COX2 antibody of the same value as ab16701. Could you please tell me which you would prefer and I will arrange this immediately, I look forward to hearing from you to arrange this,

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    Question

    The catalog number I meant to inquire about is ab16701.

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    Answer

    Thank you for your enquiry. I am not sure that I follow your question, please can you elaborate. ab16701, anti-COX2 recognizes both the rat COX2 (69164KDa) and the human COX2 (68996KDa). I hope this information helps. Please do not hesitate to contact me should you require further assistance.

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    Question

    For WB with ab16701, what type of lystae is a good positive control?

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    Answer

    Our source for this antibody has recommended HT 29 lysate as a positive control for Western blotting. This type of lysate can be obtained from Abcam; it is catalog # ab3952. If you have any additional questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com