Anti-COX2 / Cyclooxygenase 2抗体(ab52237)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-COX2 / Cyclooxygenase 2抗体
参阅全部 COX2 / Cyclooxygenase 2 一抗 -
描述
兔多克隆抗体to COX2 / Cyclooxygenase 2 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.
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阳性对照
- WB: Human primary myometrial cells whole cell lysate, A549 cells. ICC/IF: A549 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52237于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (6) |
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
|
| ICC/IF |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
|
ICC/IF
1/100 - 1/500. |
靶标
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功能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻译后修饰
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5743 Human
- Omim: 600262 Human
- SwissProt: P35354 Human
- Unigene: 196384 Human
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别名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
图片
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/1000 dilution + Human primary myometrial cells whole cell lysate at 20 µg
Secondary
Anti-rabbit IgG, HRP-linked at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial)
Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide
Predicted band size: 69 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)This image is courtesy of an anonymous AbreviewAnti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)
Secondary
Goat anti-rabbit HRP
Predicted band size: 69 kDa
Please see accompanying abreview for additional information
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (90)
ab52237 被引用在 90 文献中.
- Ghatak S et al. Identification of a Novel Five-Gene Signature as a Prognostic and Diagnostic Biomarker in Colorectal Cancers. Int J Mol Sci 23:N/A (2022). PubMed: 35054980
- Schulz MC et al. Epithelial-Fibroblast Crosstalk Protects against Acidosis-Induced Inflammatory and Fibrotic Alterations. Biomedicines 10:N/A (2022). PubMed: 35327483
- Ye Z et al. Long Noncoding RNA FOXD2-AS1 Promotes Pancreas Adenocarcinoma Cell Invasion and Migration by Sponging miR-30a-3p to Upregulate COX-2. Crit Rev Eukaryot Gene Expr 32:25-33 (2022). PubMed: 35377978
- Yang M et al. Mechanism of Traditional Tibetan Medicine Grubthobrildkr Alleviated Gastric Ulcer Induced by Acute Systemic Hypoxia in Rats. Biomed Res Int 2022:4803956 (2022). PubMed: 35425842
- Li HM et al. Polyphenols from the Peels of Punica granatum L. and Their Bioactivity of Suppressing Lipopolysaccharide-Stimulated Inflammatory Cytokines and Mediators in RAW 264.7 Cells via Activating p38 MAPK and NF-κB Signaling Pathways. Molecules 27:N/A (2022). PubMed: 35889496