Anti-COX2 / Cyclooxygenase 2抗体(ab23672)
Key features and details
- Goat polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, IHC-P
- Reacts with: Sheep, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-COX2 / Cyclooxygenase 2抗体
参阅全部 COX2 / Cyclooxygenase 2 一抗 -
描述
山羊多克隆抗体to COX2 / Cyclooxygenase 2 -
宿主
Goat -
特异性
ab23672 recognises COX2. It does not cross react with COX1. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Sheep, Human
预测可用于: Horse, Cow, Dog, Pig, Mink
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免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
(Peptide available asab49841) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab23672 was purified by affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.
|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻译后修饰
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
- SwissProt: P35354 Human
- SwissProt: P79208 Sheep
see all -
别名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
图片
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution
Lane 1 : Human COX-1
Lane 2 : Recombinant ovine COX-2 protein
Lane 3 : Recombinant human COX-2 protein
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)Immunohistochemistry analysis of formalin fixed, paraffin-embedded human kidney sections labeling COX2 / Cyclooxygenase 2 with ab23672 at 1/80 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen (DAB). Antigen retrieval was heat mediated using citrate buffer pH 6.0.
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab23672 被引用在 24 文献中.
- Kim JE et al. PLPP/CIN-mediated NF2 S10 dephosphorylation distinctly regulates kainate-induced seizure susceptibility and neuronal death through PAK1-NF-κB-COX-2-PTGES2 signaling pathway. J Neuroinflammation 20:99 (2023). PubMed: 37118736
- Zarrough AE et al. Resolvin D1 modulates periodontal ligament fibroblast function. J Periodontol 94:683-693 (2023). PubMed: 36416879
- Zang CX et al. HACE1 negatively regulates neuroinflammation through ubiquitylating and degrading Rac1 in Parkinson's disease models. Acta Pharmacol Sin 43:285-294 (2022). PubMed: 34593974
- Ouyang B et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
- Zhao Z et al. Fecal microbiota transplantation protects rotenone-induced Parkinson's disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis. Microbiome 9:226 (2021). PubMed: 34784980