重组Anti-COX1 / Cyclooxygenase 1抗体[EPR5867] (ab133319)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5867] to COX1 / Cyclooxygenase 1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-COX1 / Cyclooxygenase 1抗体[EPR5867]
参阅全部 COX1 / Cyclooxygenase 1 一抗 -
描述
兔单克隆抗体[EPR5867] to COX1 / Cyclooxygenase 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human COX1/ Cyclooxygenase 1 aa 500-600. The exact sequence is proprietary.
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阳性对照
- C2C12, A431, NIH 3T3, Neuro 2a, and L6 cell lysates; Human stomach tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 8.00 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5867 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133319于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 65-70 kDa (predicted molecular weight: 68 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 65-70 kDa (predicted molecular weight: 68 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
通路
Lipid metabolism; prostaglandin biosynthesis. -
序列相似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5742 Human
- Entrez Gene: 19224 Mouse
- Entrez Gene: 24693 Rat
- Omim: 176805 Human
- SwissProt: P23219 Human
- SwissProt: P22437 Mouse
- SwissProt: Q63921 Rat
- Unigene: 201978 Human
see all -
别名
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
图片
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : PTGS1 knockout A431 cell lysate
Lane 3 : C2C12 cell lysate
Lane 4 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab133319 was shown to bind specifically to COX1 / Cyclooxygenase 1. A band was observed at 70 kDa in wild-type A431 cell lysates with no signal observed at this size in PTGS1 knockout cell line ab270477 (knockout cell lysate ab270500). To generate this image, wild-type and PTGS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319)
Immunohistochemical analysis of Paraffin Embedded Human stomach tissue labelling COX1/Cyclooxygenase 1 with ab133319 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-COX1 / Cyclooxygenase 1 antibody [EPR5867] (ab133319) at 1/1000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : Neuro 2a cell lysate
Lane 5 : L6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab133319 被引用在 8 文献中.
- Yang M et al. Mechanism of Traditional Tibetan Medicine Grubthobrildkr Alleviated Gastric Ulcer Induced by Acute Systemic Hypoxia in Rats. Biomed Res Int 2022:4803956 (2022). PubMed: 35425842
- Nakano R et al. Non-Transcriptional and Translational Function of Canonical NF-?B Signaling in Activating ERK1/2 in IL-1ß-Induced COX-2 Expression in Synovial Fibroblasts. Front Immunol 11:579266 (2020). PubMed: 33117381
- Roul D et al. ß1-Adrenergic cardiac contractility is increased during early endotoxemic shock: Involvement of cyclooxygenases. Life Sci 236:116865 (2019). PubMed: 31525428
- Périco LL et al. Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats. World J Gastroenterol 24:4369-4383 (2018). PubMed: 30344421
- Nakano R et al. Protein kinase Ce regulates nuclear translocation of extracellular signal-regulated kinase, which contributes to bradykinin-induced cyclooxygenase-2 expression. Sci Rep 8:8535 (2018). PubMed: 29867151
- Kitanaka T et al. JNK activation is essential for activation of MEK/ERK signaling in IL-1ß-induced COX-2 expression in synovial fibroblasts. Sci Rep 7:39914 (2017). WB, RT-PCR ; Cat . PubMed: 28054591
- Hayashi G et al. Frataxin deficiency increases cyclooxygenase 2 and prostaglandins in cell and animal models of Friedreich's ataxia. Hum Mol Genet N/A:N/A (2014). WB ; Mouse, Human . PubMed: 25104852
- Qiao LN et al. Effect of Electroacupuncture Intervention on Expression of CGRP, SP, COX-1, and PGE2 of Dorsal Portion of the Cervical Spinal Cord in Rats with Neck-Incision Pain. Evid Based Complement Alternat Med 2013:294091 (2013). WB ; Rat . PubMed: 24078821