Anti-COX IV 抗体 - Mitochondrial Loading Control

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

ab16056 staining COX IV in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16056 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).Also suitable in cells fixed with 100% methanol (5 min). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• IHC-P

AbReview77631****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed human brain tissue. Stained with ab16056 at 1/150 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0

This image is courtesy of an anonymous Abreview

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

IHC image of COXIV staining in human liver FFPE section, performed on a Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16056, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• IHC-P

AbReview50943****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

ab16056 staining COX IV in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/600 ) for 12 hours at 4°C. A Cy5® donkey anti-rabbit secondary antibody was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

• IHC-P

AbReview77632****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of paraformaldehyde fixed mouse heart tissue. Stained with ab16056 at 1/500 dilution. Secondary antibody used was EnVision+ System- HRP Labelled Polymer anti rabbit. Blocking was done with 5% serum for 1 hour at 21°C. The sample was incubated with the primary antibody and 5% normal goat serum for 19 hours at 4°C. Antigen retrieval method was heat mediated Citrate pH 6.0

This image is courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

Blocking buffer : 2% BSA

Gel type : MES

Exposure Time : 1 minute

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Rat skeletal muscle tissue at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 19 kDa

Observed band size: 17 kDa

false

• WB

Unknown

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

Blocking buffer : 2% BSA

Gel type : MES

Exposure Time : 1 minute

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 19 kDa

Observed band size: 17 kDa

false

• WB

Ap

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Human liver tissue lysate - total protein (<a href='/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 3:

Human heart tissue lysate - total protein (<a href='/products/unavailable/human-heart-tissue-lysate-total-protein-ab29431'>ab29431</a>) at 10 µg

Lane 4:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab7090'>ab7090</a>) at 1/3000 dilution

Predicted band size: 19 kDa

false

• IHC-P

AbReview53575****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

ab16056 staining COX IV in breast tumour tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% buffered formalin and blocked with 5% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a 10mM sodium citrate buffer pH6. Samples were incubated with primary antibody (1/300 in blocking buffer) for 12 hours at 4°C. An Alexa Fluor^® 647-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• WB

AbReview65825****

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 1/3000 dilution

All lanes:

mouse heart whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-rabbit IgG, HRP-linked Antibody at 1/1000 dilution

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (AB16056)

All lanes:

Western blot - Anti-COX IV antibody - Mitochondrial Loading Control (ab16056) at 0.38 µg/mL

Lane 1:

Hela whole cell lysate at 20 µg

Lane 2:

Human skeletal muscle cell lysate at 20 µg

Lane 3:

Hela whole cell lysate at 20 µg with Human COX IV peptide (<a href='/products/unavailable/human-cox-iv-peptide-ab16381'>ab16381</a>)

Lane 4:

Human skeletal muscle cell lysate at 20 µg with Human COX IV peptide (<a href='/products/unavailable/human-cox-iv-peptide-ab16381'>ab16381</a>)

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution

Predicted band size: 19 kDa

Observed band size: 15 kDa

false