Anti-Cortactin (phospho Y421)抗体(ab47768)
Key features and details
- Rabbit polyclonal to Cortactin (phospho Y421)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cortactin (phospho Y421)抗体
参阅全部 Cortactin 一抗 -
描述
兔多克隆抗体to Cortactin (phospho Y421) -
宿主
Rabbit -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human Cortactin aa 350-450 (phospho Y421).
Database link: Q14247 -
阳性对照
- Hela cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47768于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa).
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说明 |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa). |
靶标
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功能
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases. -
序列相似性
Contains 7 cortactin repeats.
Contains 1 SH3 domain. -
结构域
The SH3 motif may mediate binding to the cytoskeleton. -
翻译后修饰
Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia. - Information by UniProt
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数据库链接
- Entrez Gene: 2017 Human
- Entrez Gene: 13043 Mouse
- Entrez Gene: 60465 Rat
- GenBank: NM_138565 Human
- GenBank: NM_007803 Mouse
- Omim: 164765 Human
- SwissProt: Q14247 Human
- SwissProt: Q60598 Mouse
see all -
别名
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
see all
图片
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All lanes : Anti-Cortactin (phospho Y421) antibody (ab47768) at 1/500 dilution
Lane 1 : Hela cell extract, treated with H2O2
Lane 2 : Hela cell extract, untreated
Predicted band size: 62 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. The cells were treated with 150 uM H2O2.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab47768 被引用在 9 文献中.
- An S et al. SAMHD1-induced endosomal FAK signaling promotes human renal clear cell carcinoma metastasis by activating Rac1-mediated lamellipodia protrusion. Exp Mol Med 55:779-793 (2023). PubMed: 37009792
- Jones NM et al. Cortactin loss protects against hemin-induced acute lung injury in sickle cell disease. Am J Physiol Lung Cell Mol Physiol 322:L890-L897 (2022). PubMed: 35503995
- Masi I et al. Endothelin-1 drives invadopodia and interaction with mesothelial cells through ILK. Cell Rep 34:108800 (2021). PubMed: 33657382
- Zhou Z et al. Autophagy promotes invadopodia formation in human ovarian cancer cells via the p62-extracellular signal-regulated kinase 1/2 pathway. Exp Ther Med 22:952 (2021). PubMed: 34335894
- Liu Y et al. Arginine methylation of SHANK2 by PRMT7 promotes human breast cancer metastasis through activating endosomal FAK signalling. Elife 9:N/A (2020). PubMed: 32844749
- Klomp JE et al. Time-Variant SRC Kinase Activation Determines Endothelial Permeability Response. Cell Chem Biol 26:1081-1094.e6 (2019). PubMed: 31130521
- Chellini L et al. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancer. Matrix Biol 81:17-33 (2019). PubMed: 30367951
- Meiler E et al. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS One 7:e33662 (2012). WB . PubMed: 22479425
- García-Castillo J et al. HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation. J Biol Chem 284:25302-13 (2009). WB ; Human . PubMed: 19589785