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使用敲除细胞株进行验证重组RabMAb

重组Anti-Cortactin抗体[EP1922Y] - BSA and Azide free (ab223125)

Submit a review Submit a question References (4)
Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
  • Immunoprecipitation - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
  • Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
  • Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1922Y] to Cortactin - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 PE Unconjugated

概述

  • 产品名称

    Anti-Cortactin抗体[EP1922Y] - BSA and Azide free
    参阅全部 Cortactin 一抗

  • 描述

    兔单克隆抗体[EP1922Y] to Cortactin - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: MCF7 and wildtype HAP1 cells. IP: HeLa cell lysate

  • 常规说明

    ab223125 is the carrier-free version of ab81208.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223125于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.

  • 序列相似性

    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.

  • 结构域

    The SH3 motif may mediate binding to the cytoskeleton.

  • 翻译后修饰

    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.

  • 细胞定位

    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

    ab81208 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab81208 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81208).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

    Immunohistochemical staining of paraffin-embedded human breast carcinoma using 1/100 ab81208.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81208).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Immunoprecipitation - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

    Purified ab81208 at 1/50 dilution (2µg) immunoprecipitating Cortactin in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab81208 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab81208 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 62 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81208).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Immunofluorescent staining of MCF7 cells using 1/100 ab81208

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81208).

  • Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Flow Cytometry (Intracellular) - Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

    Overlay histogram showing HeLa cells stained with ab81208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81208, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81208). 

     

     

  • Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
    Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)

文献 (4)

发表研究结果有使用 ab223125？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab223125 被引用在 4 文献中.

  • Motonishi S  et al. Sirtuin1 Maintains Actin Cytoskeleton by Deacetylation of Cortactin in Injured Podocytes. J Am Soc Nephrol 26:1939-59 (2015). PubMed: 25424328
  • Kaur S  et al. An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis. Elife 3:e00829 (2014). ICC/IF ; Human . PubMed: 24550251
  • Huang X  et al. O-GlcNAcylation of cofilin promotes breast cancer cell invasion. J Biol Chem 288:36418-25 (2013). WB, ICC/IF ; Human . PubMed: 24214978
  • Hirooka S  et al. Localization of the invadopodia-related proteins actinin-1 and cortactin to matrix-contact-side cytoplasm of cancer cells in surgically resected lung adenocarcinomas. Pathobiology 78:10-23 (2011). IHC ; Human . PubMed: 21474972

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