Anti-Coronin 1a/TACO抗体(ab72212)
Key features and details
- Rabbit polyclonal to Coronin 1a/TACO
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Coronin 1a/TACO抗体
参阅全部 Coronin 1a/TACO 一抗 -
描述
兔多克隆抗体to Coronin 1a/TACO -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
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免疫原
Synthetic peptide corresponding to Human Coronin 1a/TACO.
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阳性对照
- Jurkat whole cell lysate (ab7899)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72212于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. -
组织特异性
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. -
序列相似性
Belongs to the WD repeat coronin family.
Contains 7 WD repeats. -
细胞定位
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. - Information by UniProt
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数据库链接
- Entrez Gene: 11151 Human
- Omim: 605000 Human
- SwissProt: P31146 Human
- Unigene: 415067 Human
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别名
- Actin binding protein antibody
- CLABP antibody
- Clipin A antibody
see all
图片
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Western blot - Anti-Coronin 1a/TACO antibody (ab72212)All lanes : Anti-Coronin 1a/TACO antibody (ab72212) at 0.04 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody (ab72212)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Coronin 1a/TACO with ab72212 at 1/1000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Coronin 1a/TACO antibody (ab72212)Immunoprecipitation analysis of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded). ab72212 used for IP at 3 µg/mg lysate and for WB at 1 µg/ml. Lane 1 - ab72212 IP. Lane 2 - control IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab72212 被引用在 3 文献中.
- Riley DRJ et al. Coronin 1 Is Required for Integrin ß2 Translocation in Platelets. Int J Mol Sci 21:N/A (2020). PubMed: 31948107
- Qiao X et al. Wnt5a regulates Ameloblastoma Cell Migration by modulating Mitochondrial and Cytoskeletal Dynamics. J Cancer 11:5490-5502 (2020). PubMed: 32742496
- Riley DRJ et al. Biochemical and immunocytochemical characterization of coronins in platelets. Platelets N/A:1-12 (2019). PubMed: 31801396