重组Anti-Corin抗体[EPR23361-77] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] - BSA and Azide free (AB274323)

This data was developed using ab274320, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Corin with ab274320 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cardiac muscle (PMID : 11082206). The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] - BSA and Azide free (AB274323)

This data was developed using ab274320, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Corin with ab274320 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney. The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-Corin antibody [EPR23361-77] - BSA and Azide free (AB274323)

This data was developed using ab274320, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Tissue specific protein, highly expressed in heart (PMID : 11082006).

The expression profile observed is consistent with what has been described in the literature (PMID : 29387201, 17996891).

Low expression : human brain (PMID : 11082006).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Corin antibody [EPR23361-77] (<a href='/products/primary-antibodies/corin-antibody-epr23361-77-ab274320'>ab274320</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 116 kDa

Observed band size: 100-180 kDa

false

• WB

Lab

Western blot - Anti-Corin antibody [EPR23361-77] - BSA and Azide free (AB274323)

This data was developed using ab274320, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Tissue specific protein, highly expressed in heart (PMID : 11082006).

The expression profile observed is consistent with what has been described in the literature (PMID : 29387201, 17996891).

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Corin antibody [EPR23361-77] (<a href='/products/primary-antibodies/corin-antibody-epr23361-77-ab274320'>ab274320</a>) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 116 kDa

Observed band size: 100-180 kDa

false