重组Anti-Corin抗体[EPR23361-77] (ab274320)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)](https://www.abcam.cn/ps/products/274/ab274320/Images/ab274320-1-anti-corin-antibody-epr2336177-immunohistochemistry-human-cardiac-muscle.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23361-77] to Corin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Corin抗体[EPR23361-77]
参阅全部 Corin 一抗 -
描述
兔单克隆抗体[EPR23361-77] to Corin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human and mouse heart tissue lysate. IHC-P: Human cardiac muscle and kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23361-77 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab274320于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100-180 kDa (predicted molecular weight: 116 kDa).
|
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 100-180 kDa (predicted molecular weight: 116 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Serine-type endopeptidase involved in atrial natriuretic peptide hormone processing. Converts through proteolytic cleavage the non-functional propeptide NPPA/ANP into the active hormone which promotes natriuresis, diuresis, and vasodilatation. May also process pro-NPPB the B-type natriuretic peptide. -
组织特异性
Highly expressed in heart. Expressed in heart myocytes. -
序列相似性
Belongs to the peptidase S1 family.
Contains 2 FZ (frizzled) domains.
Contains 7 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain. -
翻译后修饰
N-glycosylated; required for processing and activation.
Activated through proteolytic processing by a trypsin-like protease; cleaved into a N-terminal propeptide and an activated corin protease fragment.
A disulfide bond links the N-terminal propeptide and the activated corin protease fragment. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10699 Human
- Entrez Gene: 53419 Mouse
- Omim: 605236 Human
- SwissProt: Q9Y5Q5 Human
- SwissProt: Q9Z319 Mouse
- Unigene: 518618 Human
- Unigene: 332425 Mouse
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别名
- activated protease fragment antibody
- ATC 2 antibody
- ATC2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cardiac muscle (PMID: 11082206). The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)](https://www.abcam.cn/ps/products/274/ab274320/Images/ab274320-397327-anti-corin-antibody-epr2336177-western-blot-mouse-heart-tissue-lysate20-ug.jpg)
Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution + Mouse heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 116 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Tissue specific protein, highly expressed in heart (PMID: 11082006).
The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).
Exposure time: 10 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)](https://www.abcam.cn/ps/products/274/ab274320/Images/ab274320-2-anti-corin-antibody-epr2336177-immunohistochemistry-human-kidney.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Corin antibody [EPR23361-77] (ab274320)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Corin with ab274320 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human kidney. The section was incubated with ab274320 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)](https://www.abcam.cn/ps/products/274/ab274320/Images/ab274320-397326-anti-corin-antibody-epr2336177-western-blot-human-heart-tissue-lysate20-ug.jpg)
Western blot - Anti-Corin antibody [EPR23361-77] (ab274320)All lanes : Anti-Corin antibody [EPR23361-77] (ab274320) at 1/1000 dilution
Lane 1 : Human heart tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 116 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Tissue specific protein, highly expressed in heart (PMID: 11082006).
The expression profile observed is consistent with what has been described in the literature (PMID: 29387201, 17996891).
Low expression: human brain (PMID: 11082006).
Exposure time: 3 minutes.
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![Anti-Corin antibody [EPR23361-77] (ab274320)](https://www.abcam.cn/ps/products/274/ab274320/Images/ab274320-5-benefits-of-recombinant-antibodies.png)
Anti-Corin antibody [EPR23361-77] (ab274320)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab274320 被引用在 1 文献中.
- Chu N et al. ANP promotes HTR-8/SVneo cell invasion by upregulating protein kinase N 3 via autophagy inhibition. FASEB J 37:e22779 (2023). PubMed: 36723798