重组Anti-COPS3/CSN3抗体[EPR3127] (ab79698)
![Western blot - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)](https://www.abcam.cn/ps/products/79/ab79698/Images/ab79698-95155-anti-cops3-antibody-epr3127-western-blot.jpg "Western blot - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3127] to COPS3/CSN3
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-COPS3/CSN3抗体[EPR3127]
参阅全部 COPS3/CSN3 一抗 -
描述
兔单克隆抗体[EPR3127] to COPS3/CSN3 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide within Human COPS3/CSN3 aa 400-500 (C terminal). The exact sequence is proprietary.
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阳性对照
- HT29 cell lysate HeLa cells SKBR-3 cell lysate 293T cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3127 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab79698于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/20 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
|
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| ICC/IF |
1/250 - 1/500.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/20 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
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ICC/IF
1/250 - 1/500. |
靶标
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功能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
组织特异性
Widely expressed. Expressed at high level in heart and skeletal muscle. -
序列相似性
Belongs to the CSN3 family.
Contains 1 PCI domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8533 Human
- Entrez Gene: 26572 Mouse
- Entrez Gene: 287367 Rat
- Omim: 604665 Human
- SwissProt: Q9UNS2 Human
- SwissProt: O88543 Mouse
- SwissProt: Q68FW9 Rat
- Unigene: 6076 Human
see all -
别名
- Constitutive photomorphogenic homolog subunit 3 antibody
- COP 9 (constitutive photomorphogenic) subunit 3 antibody
- COP 9 complex homolog subunit 3 antibody
see all
图片
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Western blot - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)All lanes : Anti-COPS3/CSN3 antibody [EPR3127] (ab79698) at 1/20000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)](https://www.abcam.cn/ps/products/79/ab79698/Images/ab79698-263493-anti-cops3-antibody-epr3127-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)Immunofluorescence staining of HeLa cells with purified ab79698 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
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![Flow Cytometry (Intracellular) - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)](https://www.abcam.cn/ps/products/79/ab79698/Images/ab79698-95153-anti-cops3-antibody-epr3127-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79698, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)](https://www.abcam.cn/ps/products/79/ab79698/Images/ab79698-5-benefits-of-recombinant-antibodies.png)
Anti-COPS3/CSN3 antibody [EPR3127] (ab79698)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab79698 被引用在 10 文献中.
- Dubiel D et al. Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18. iScience 26:106468 (2023). PubMed: 37091236
- Füzesi-Levi MG et al. The C-terminal tail of CSNAP attenuates the CSN complex. Life Sci Alliance 6:N/A (2023). PubMed: 37460146
- Lin H et al. IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion. Nat Commun 12:2461 (2021). PubMed: 33911083
- Pariano M et al. Anakinra Activates Superoxide Dismutase 2 to Mitigate Inflammasome Activity. Int J Mol Sci 22:N/A (2021). PubMed: 34207085
- Boro M et al. Thrombin-Par1 signaling axis disrupts COP9 signalosome subunit 3-mediated ABCA1 stabilization in inducing foam cell formation and atherogenesis. Cell Death Differ 28:780-798 (2021). PubMed: 32968199
- Zarich N et al. The CSN3 subunit of the COP9 signalosome interacts with the HD region of Sos1 regulating stability of this GEF protein. Oncogenesis 8:2 (2019). PubMed: 30631038
- Füzesi-Levi MG et al. CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases. Cell Death Differ N/A:N/A (2019). PubMed: 31367012
- Zhang F et al. Novel oncogene COPS3 interacts with Beclin1 and Raf-1 to regulate metastasis of osteosarcoma through autophagy. J Exp Clin Cancer Res 37:135 (2018). PubMed: 29970115
- Füzesi-Levi MG et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . PubMed: 24421388
- Korczeniewska J & Barnes BJ The COP9 signalosome interacts with and regulates interferon regulatory factor 5 protein stability. Mol Cell Biol 33:1124-38 (2013). PubMed: 23275442