Anti-COPS3/CSN3抗体(ab12321)
Key features and details
- Rabbit polyclonal to COPS3/CSN3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-COPS3/CSN3抗体
参阅全部 COPS3/CSN3 一抗 -
描述
兔多克隆抗体to COPS3/CSN3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus
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免疫原
Synthetic peptide within Human COPS3/CSN3 aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UNS2 -
阳性对照
- HEK 293T cells.
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常规说明
Centrifuge tube to remove product from lid.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified using the peptide immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab12321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/25000. Predicted molecular weight: 51 kDa.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/25000. Predicted molecular weight: 51 kDa. |
靶标
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功能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
组织特异性
Widely expressed. Expressed at high level in heart and skeletal muscle. -
序列相似性
Belongs to the CSN3 family.
Contains 1 PCI domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 416504 Chicken
- Entrez Gene: 507932 Cow
- Entrez Gene: 479528 Dog
- Entrez Gene: 8533 Human
- Entrez Gene: 26572 Mouse
- Entrez Gene: 100171418 Orangutan
- Entrez Gene: 287367 Rat
- Entrez Gene: 379813 Xenopus laevis
see all -
别名
- Constitutive photomorphogenic homolog subunit 3 antibody
- COP 9 (constitutive photomorphogenic) subunit 3 antibody
- COP 9 complex homolog subunit 3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (ab12321)
Immunohisotchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling COPS3/CSN3 with ab12321 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-COPS3/CSN3 antibody (ab12321)All lanes : Anti-COPS3/CSN3 antibody (ab12321) at 0.04 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 51 kDa
Exposure time: 10 seconds -
Western blot - Anti-COPS3/CSN3 antibody (ab12321)Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab12321 被引用在 2 文献中.
- Olma MH et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 122:1035-44 (2009). WB ; Human . PubMed: 19295130
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822