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Anti-COP1抗体(ab70889)

Submit a review Q&A (1)References (1)
Western blot - Anti-COP1 antibody (ab70889)
  • Immunoprecipitation - Anti-COP1 antibody (ab70889)

Key features and details

  • Rabbit polyclonal to COP1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-COP1抗体
    参阅全部 COP1 一抗

  • 描述

    兔多克隆抗体to COP1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • 免疫原

    A region between residue 1 and 50 of human COP1. (NP_071902.2)

  • 阳性对照

    • HeLa, 293T and NIH3T3 whole cell lysates.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab70889于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IP
Use a concentration of 2 - 5 µg/ml.
说明
WB
1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IP
Use a concentration of 2 - 5 µg/ml.

靶标

  • 功能

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

  • 组织特异性

    Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.

  • 通路

    Protein modification; protein ubiquitination.

  • 序列相似性

    Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.

  • 结构域

    The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).

  • 细胞定位

    Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Constitutive photomorphogenesis protein 1 homolog antibody
      • COP antibody
      • E3 ubiquitin-protein ligase RFWD2 antibody
      • hCOP1 antibody
      • Rfwd2 antibody
      • RFWD2_HUMAN antibody
      • ring finger and WD repeat domain 2 antibody
      • RING finger and WD repeat domain protein 2 antibody
      • RING finger protein 200 antibody
      see all

    图片

    • Western blot - Anti-COP1 antibody (ab70889)
      Western blot - Anti-COP1 antibody (ab70889)
      All lanes : Anti-COP1 antibody (ab70889) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 80 kDa
      Observed band size: 80 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-COP1 antibody (ab70889)
      Immunoprecipitation - Anti-COP1 antibody (ab70889)
      HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    文献 (1)

    发表研究结果有使用 ab70889？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab70889 被引用在 1 文献中.

    • Ren X  et al. TRB3 stimulates SIRT1 degradation and induces insulin resistance by lipotoxicity via COP1. Exp Cell Res 382:111428 (2019). PubMed: 31125554

    客户评价及客户问答

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    Question

    Hello, I was wondering if you could tell me what a good negative control (cell line) would be for Anti-COP1 antibody (ab70889) and also if you know that it will pick up tagged forms of overexpressed COP1. Please let me know. Thanks so much.

    Read More
    Answer

    Thank you for contacting Abcam.

    The antibody ab70889, should recognize both overexpressed and endogenous forms of the protein. As for negative controls, our notes indicate that the protein is ubiquitously expressed at low level and expressed at higher level in testis, placenta, skeletal muscle and heart. Unfortunately, I cannot say for sure what would be a good negative control; therefore, I would recommend that you do a literature search to see what others in the field have used.

    If there is anything else I can help you with, please let me know.

    Read More

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