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Anti-COP1抗体[1E4] (ab56400)

Reviews (4)Q&A (1)References (15)
Western blot - Anti-COP1 antibody [1E4] (ab56400)
  • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)
  • Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)

Key features and details

  • Mouse monoclonal [1E4] to COP1
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-COP1抗体[1E4]
    参阅全部 COP1 一抗

  • 描述

    小鼠单克隆抗体[1E4] to COP1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IF, IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Mouse, Human

  • 免疫原

    Recombinant fragment: CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV , corresponding to amino acids 632-732 of Human COP1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab56400于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
ICC/IF (1)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

靶标

  • 功能

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

  • 组织特异性

    Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.

  • 通路

    Protein modification; protein ubiquitination.

  • 序列相似性

    Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.

  • 结构域

    The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).

  • 细胞定位

    Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Constitutive photomorphogenesis protein 1 homolog antibody
      • COP antibody
      • E3 ubiquitin-protein ligase RFWD2 antibody
      • hCOP1 antibody
      • Rfwd2 antibody
      • RFWD2_HUMAN antibody
      • ring finger and WD repeat domain 2 antibody
      • RING finger and WD repeat domain protein 2 antibody
      • RING finger protein 200 antibody
      see all

    图片

    • Western blot - Anti-COP1 antibody [1E4] (ab56400)
      Western blot - Anti-COP1 antibody [1E4] (ab56400)

      COP1 antibody (ab56400) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)
      Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)

      Immunofluorescence of ab56400 on NIH/3T3 cell [antibody concentration 10 ug/ml].

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)
      Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)

      Overlay histogram showing NIH3T3 cells stained with ab56400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56400, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)

      IHC image of COP1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      This image was generated using the ascites version of the product.

    文献 (15)

    发表研究结果有使用 ab56400？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab56400 被引用在 15 文献中.

    • Albino D  et al. Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer. Commun Biol 4:119 (2021). PubMed: 33500545
    • Guo M  et al. Targeting RFWD2 as an Effective Strategy to Inhibit Cellular Proliferation and Overcome Drug Resistance to Proteasome Inhibitor in Multiple Myeloma. Front Cell Dev Biol 9:675939 (2021). PubMed: 33968945
    • Ding L  et al. Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis. Nat Metab 3:1648-1661 (2021). PubMed: 34903883
    • Yoshikawa R  et al. Development of synthetic microRNA-214 showing enhanced cytotoxicity and RNase resistance for treatment of canine hemangiosarcoma. Vet Comp Oncol 18:570-579 (2020). PubMed: 32072720
    • Xu Y  et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024
    View all Publications for this product

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-COP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BEL-7402)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    40 µg
    Treatment
    80 µM Doxorubicin for 24hrs
    Specification
    BEL-7402
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. guoqing zhu

    Verified customer

    提交于 Jan 22 2016

    Western blot abreview for Anti-COP1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Loading amount
    20 µg
    Specification
    kidney
    Gel Running Conditions
    Reduced Denaturing (10-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 11 2013

    Western blot abreview for Anti-COP1 antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cell)
    Loading amount
    50 µg
    Specification
    293T cell
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 08 2012

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