AB270278

Anti-Connexin 32 / GJB1抗体[M12.13] - BSA and Azide free

Name: Connexin 32 / GJB1抗体[M12.13] - BSA and Azide free (ab270278)| Abcam中文官网
Brand: Abcam Limited
SKU: AB270278
Price: 9609 CNY
Availability: InStock

Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free

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Mouse Monoclonal Connexin 32 / GJB1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Rat Gjb1.

查看别名

Cxn-32, Gap junction beta-1 protein, Connexin-32, GAP junction 28 kDa liver protein, Cx32

1 Images

Supplier Data

Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (AB270278)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270241).

All lanes:

Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (ab270278) at 2 µg/mL

All lanes:

Human stomach lysate

Predicted band size: 32 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

M12.13

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

Native Full Length Protein corresponding to Rat Gjb1.

P08033

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab270278 is a carrier free version of ab270241.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purified from bioreactor concentrate.

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 32 also known as GJB1 is a gap junction protein with a molecular mass of approximately 32 kDa. This protein facilitates direct intercellular communication by forming gap junction channels that allow ions and small molecules to pass between adjacent cells. Connexin 32 is expressed in various tissues including liver pancreas and many types of epithelial cells. Its expression ensures cells can synchronize their activities important for maintaining tissue homeostasis.

Biological function summary

Connexin 32 plays a significant role in maintaining cellular communication networks. This protein forms hexameric complexes called connexons which dock with connexons of adjacent cells to create functional gap junction channels. These channels are essential in processes such as maintaining homeostasis cell differentiation and synchronized contraction of tissues like cardiac muscle. The structural formation allows direct transfer of metabolites and signaling molecules highlighting its importance in multicellular organism function.

Pathways

Connexin 32 actively contributes to cellular communication and metabolic coordination. It participates in the phosphoinositide 3-kinase (PI3-K) pathway and the ERK/MAPK signaling pathway which are important for cell growth proliferation and apoptosis. Connexin 32 interacts with proteins like Connexin 43 with both proteins playing roles in channel assembly and intracellular signaling processes that influence cellular responses to external stimuli.

Connexin 32's dysfunction aligns with specific neuropathies. Mutations in the GJB1 gene often lead to X-linked Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. This disease involves degeneration of nerve fibers disrupting muscle function and sensation and connects with proteins that share functional pathways like PMP22. Additionally Connexin 32 has implications in liver diseases where its reduced expression correlates with disrupted hepatocyte communication affecting liver function and regeneration.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

See full target information Gjb1

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Anti-Connexin 32 / GJB1抗体[M12.13] - BSA and Azide free

Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free

Western blot - Anti-Connexin 32 / GJB1 antibody [M12.13] - BSA and Azide free (AB270278)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原