Anti-Connecdenn 3抗体(ab224721)
Key features and details
- Rabbit polyclonal to Connecdenn 3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-Connecdenn 3抗体 -
描述
兔多克隆抗体to Connecdenn 3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment corresponding to Human Connecdenn 3 aa 600 to the C-terminus.
Database link: Q8IV53 -
阳性对照
- WB: Rat liver and lung lysates. IHC-P: Human lymph node and lung tissues.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 87 kDa).
|
|
| IHC-P |
1/20 - 1/200.
|
| 说明 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 87 kDa). |
|
IHC-P
1/20 - 1/200. |
靶标
-
功能
Guanine nucleotide exchange factor (GEF) for RAB35. -
序列相似性
Contains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain. -
细胞定位
Cytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle. - Information by UniProt
-
数据库链接
- Entrez Gene: 79958 Human
- Omim: 613634 Human
- SwissProt: Q8IV53 Human
- Unigene: 236449 Human
-
别名
- Connecdenn 3 antibody
- DEN1C_HUMAN antibody
- DENN domain containing 1C antibody
see all
图片
-
Western blot - Anti-Connecdenn 3 antibody (ab224721)All lanes : Anti-Connecdenn 3 antibody (ab224721) at 1/500 dilution
Lane 1 : Rat liver lysate
Lane 2 : Rat lung lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (ab224721)Paraffin-embedded human lymph node tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connecdenn 3 antibody (ab224721)Paraffin-embedded human lung tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab224721 尚未被引用在任何文献中。