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使用敲除细胞株进行验证重组RabMAb

重组Anti-COMT抗体[EPR6490] (ab126618)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (7)

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Western blot - Anti-COMT antibody [EPR6490] (ab126618)
  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
  • Anti-COMT antibody [EPR6490] (ab126618)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6490] to COMT
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Human COMT knockout HEK-293T cell lysate (ab257396)

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概述

  • 产品名称

    Anti-COMT抗体[EPR6490]
    参阅全部 COMT 一抗
  • 描述

    兔单克隆抗体[EPR6490] to COMT
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
    不适用于: IHC-P
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human COMT. Human COMT is a soluble isoform.
    Database link: P21964

  • 阳性对照

    • WB: HEK-293T, HAP1, A431, MOLT4 and MCF7 cell lysates; Human fetal liver lysate. IHC-P: Human liver and kidney tissues.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR6490
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Alternative Versions

    • Anti-COMT antibody [EPR6490] - BSA and Azide free (ab248128)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human COMT knockout HEK-293T cell line (ab266537)
  • KO cell lysates

    • Human COMT knockout HEK-293T cell lysate (ab257396)
  • Positive Controls

    • A-431 membrane extract lysate (ab30134)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab126618于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
说明
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
应用说明
Is unsuitable for IHC-P.

靶标

  • 功能

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
  • 组织特异性

    Brain, liver, placenta, lymphocytes and erythrocytes.
  • 序列相似性

    Belongs to the mammalian catechol-O-methyltransferase family.
  • 翻译后修饰

    The N-terminus is blocked.
  • 细胞定位

    Cytoplasm and Cell membrane.
  • Target information above from: UniProt accession P21964 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1312 Human
    • Entrez Gene: 12846 Mouse
    • Entrez Gene: 24267 Rat
    • Omim: 116790 Human
    • SwissProt: P21964 Human
    • SwissProt: O88587 Mouse
    • SwissProt: P22734 Rat
    • Unigene: 370408 Human
    • Unigene: 704514 Human
    • Unigene: 100940 Mouse
    • Unigene: 220 Rat
    see all
  • 别名

    • Catechol O methyltransferase antibody
    • Catechol O-methyltransferase antibody
    • COMT antibody
    • COMT_HUMAN antibody
    • EC 2.1.1.6 antibody
    see all

图片

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution (Purified)

    Lane 1 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Rat kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 30 kDa

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : COMT knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 30 kDa
    Observed band size: 24-28 kDa why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab126618 observed at 24-28 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab126618 Anti-COMT antibody [EPR6490] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab126618 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    Western blot - Anti-COMT antibody [EPR6490] (ab126618)
    All lanes : Anti-COMT antibody [EPR6490] (ab126618) at 1/1000 dilution

    Lane 1 : Wild type HAP1 whole cell lysate at 40 µg
    Lane 2 : COMT knockout HAP1 whole cell lysate at 40 µg
    Lane 3 : A431 whole cell lysate at 20 µg
    Lane 4 : MCF7 whole cell lysate at 20 µg

    Predicted band size: 30 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126618 observed at 24/28 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab126618 was shown to specifically react with COMT when COMT knockout samples were used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Samples were incubated with ab126618 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at 1/1000 and 1/10000 dilutions respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-COMT antibody [EPR6490] (ab126618)
    Anti-COMT antibody [EPR6490] (ab126618)

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (7)

发表研究结果有使用 ab126618?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab126618 被引用在 7 文献中.

  • Valsecchi V  et al. SMN deficiency perturbs monoamine neurotransmitter metabolism in spinal muscular atrophy. Commun Biol 6:1155 (2023). PubMed: 37957344
  • Ng J  et al. Gene therapy restores dopamine transporter expression and ameliorates pathology in iPSC and mouse models of infantile parkinsonism. Sci Transl Med 13:N/A (2021). PubMed: 34011628
  • Cooper TK  et al. Coati Bodies: Cytoplasmic Hyaline Globules in the Ganglionic Neurons of Aging Captive Coatis. Vet Pathol 54:851-854 (2017). PubMed: 28578627
  • Winner BM  et al. Metabolism of Dopamine in Nucleus Accumbens Astrocytes Is Preserved in Aged Mice Exposed to MPTP. Front Aging Neurosci 9:410 (2017). PubMed: 29311899
  • Doan KV  et al. FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase. Nat Commun 7:12733 (2016). PubMed: 27681312
  • Stefanovic B  et al. Melatonin mediated antidepressant-like effect in the hippocampus of chronic stress-induced depression rats: Regulating vesicular monoamine transporter 2 and monoamine oxidase A levels. Eur Neuropsychopharmacol 26:1629-37 (2016). WB ; Rat . PubMed: 27499503
  • Wakuda T  et al. Perinatal asphyxia alters neuregulin-1 and COMT gene expression in the medial prefrontal cortex in rats. Prog Neuropsychopharmacol Biol Psychiatry 56:149-54 (2015). PubMed: 25194460

客户评价及客户问答

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Immunohistochemistry (Frozen sections) abreview for Anti-COMT antibody [EPR6490]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 25 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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