Anti-Collagen X抗体[COL-10] (ab49945)
Key features and details
- Mouse monoclonal [COL-10] to Collagen X
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgM
概述
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产品名称
Anti-Collagen X抗体[COL-10]
参阅全部 Collagen X 一抗 -
描述
小鼠单克隆抗体[COL-10] to Collagen X -
宿主
Mouse -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Purified pig Collagen type X.
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表位
Hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. -
阳性对照
- IHC-P: Enzyme treated sections of decalcified human osteochondroma.
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常规说明
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituent: 0.142% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
COL-10 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab49945于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (3) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
|
说明 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Type X collagen is a product of hyperthrophic chondrotocytes and has been localized to presumptive mineralization zones of hyaline cartilage. -
疾病相关
Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]. SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. -
序列相似性
Contains 1 C1q domain. -
翻译后修饰
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 1300 Human
- Omim: 120110 Human
- SwissProt: Q03692 Human
- Unigene: 520339 Human
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别名
- COAA1_HUMAN antibody
- Col10a 1 antibody
- COL10A1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen X antibody [COL-10] (ab49945)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ostochondroma tissue labelling Collagen X with ab49945 at a dilution of 1/1000.
This image was generated using the ascites version of the product.
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ICC/IF of HT1080 human fibrosarcoma cells labelling Collagen X with ab49945 at 1/1000 dilution. The cells were methanol acetone fixed. Secondary antibody was a Cy3 anti mouse IgG. Nuclei are stained with DAPI.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (99)
ab49945 被引用在 99 文献中.
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). IHC-Fr ; Mouse . PubMed: 37692479
- Vinikoor T et al. Injectable and biodegradable piezoelectric hydrogel for osteoarthritis treatment. Nat Commun 14:6257 (2023). PubMed: 37802985
- Li Y et al. O-alg-THAM/gel hydrogels functionalized with engineered microspheres based on mesenchymal stem cell secretion recruit endogenous stem cells for cartilage repair. Bioact Mater 28:255-272 (2023). PubMed: 37303853
- Li T et al. Regeneration of Humeral Head Using a 3D Bioprinted Anisotropic Scaffold with Dual Modulation of Endochondral Ossification. Adv Sci (Weinh) 10:e2205059 (2023). PubMed: 36755334
- Zhou T et al. Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution. Adv Sci (Weinh) 10:e2206296 (2023). PubMed: 36965031