Anti-Collagen V抗体(ab7046)
Key features and details
- Rabbit polyclonal to Collagen V
- Suitable for: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICC
- Reacts with: Mouse, Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-Collagen V抗体
参阅全部 Collagen V 一抗 -
描述
兔多克隆抗体to Collagen V -
宿主
Rabbit -
特异性
Negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. -
经测试应用
适用于: WB, Sandwich ELISA, IHC-P, IP, IHC-Fr, ICCmore details -
种属反应性
与反应: Mouse, Cow, Human -
免疫原
Full length native protein (purified) corresponding to Collagen V aa 1-1745.
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常规说明
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 4.8% Sodium borate, 0.15% EDTA, 0.44% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
|
| IHC-P | (6) |
1/50 - 1/200.
|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| ICC |
Use at an assay dependent concentration. PubMed: 18385800
|
| 说明 |
|---|
|
WB
1/5000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 180 kDa). Native conditions are recommended. |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (ab6582). |
|
IHC-P
1/50 - 1/200. |
|
IP
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. PubMed: 18385800 |
靶标
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功能
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. -
疾病相关
Ehlers-Danlos syndrome 1
Ehlers-Danlos syndrome 2 -
序列相似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 laminin G-like domain. -
结构域
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
翻译后修饰
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Sulfated on 40% of tyrosines. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 282192 Cow
- Entrez Gene: 1289 Human
- Entrez Gene: 1290 Human
- Entrez Gene: 50509 Human
- Entrez Gene: 12831 Mouse
- Entrez Gene: 12832 Mouse
- Entrez Gene: 53867 Mouse
- Omim: 120190 Human
see all -
别名
- AB collagen antibody
- Alpha 1 type V collagen antibody
- Alpha 2 type V collagen antibody
see all
图片
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Western blot - Anti-Collagen V antibody (ab7046)Anti-Collagen V antibody (ab7046) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 180 kDa
Additional bands at: 122 kDa, 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)This image is courtesy of an anonymous Abreview.ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (ab7046)Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100ºC for 20 mins.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab7046 被引用在 50 文献中.
- Maamra M et al. Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling. Br J Pharmacol 179:2697-2712 (2022). PubMed: 34879432
- Sterken BA et al. C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells. NPJ Breast Cancer 8:11 (2022). PubMed: 35042889
- Alhayek A et al. Inhibition of Collagenase Q1 of Bacillus cereus as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections. Adv Ther (Weinh) 5:2100222 (2022). PubMed: 35310821
- Roberts E et al. Cell contractility and Focal Adhesion Kinase control circumferential arterial stiffness. Vasc Biol 4:28-39 (2022). PubMed: 36222505
- Chandrasekaran P et al. Type V collagen regulates the structure and biomechanics of TMJ condylar cartilage: A fibrous-hyaline hybrid. Matrix Biol 102:1-19 (2021). PubMed: 34314838