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AB7046

Anti-Collagen V 抗体

Anti-Collagen V antibody

5

(11 Reviews)

|

(56 Publications)

Anti-Collagen V antibody (ab7046) is a rabbit polyclonal antibody detecting Collagen V in Western Blot, IP, IHC-P, IHC-Fr, ELISA. Suitable for Cow, Human, Mouse.

- Over 50 publications
- Trusted since 2001

查看别名

Collagen alpha-1(V) chain, COL5A1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB7046)
  • IHC-P

AbReview27228****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB7046)

ab7046 staining Collagen V in Human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formaldehyde and subjected to heat-mediated antigen retrieval in citrate pH 6 prior to blocking with 0.25% casein for 5 minutes at 25°C. The primary antibody was diluted 1/75 in Tris--HCL and incubated with the sample for 30 minutes at 25°C. An HRP polymer-conjugated goat anti-rabbit antibody was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

Western blot - Anti-Collagen V antibody (AB7046)
  • WB

Unknown

Western blot - Anti-Collagen V antibody (AB7046)

All lanes:

Western blot - Anti-Collagen V antibody (ab7046) at 1 µg/mL

All lanes:

Human pancreas tissue lysate - total protein (<a href='/products/unavailable/human-pancreas-tissue-lysate-total-protein-ab29816'>ab29816</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 184 kDa

Observed band size: 122 kDa,180 kDa,54 kDa

true

Exposure time: 8min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB7046)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen V antibody (AB7046)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human lung (left) and placenta (right) sections labelling collagen V with ab7046 at a dilution of 1/200 for 45 minutes at room temperature. An antigen retrival step was performed with 0.01 M sodium citrate buffer pH 6.0 at 100°C for 20 mins.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse, Cow

应用

sELISA, WB, ICC, IHC-P, IP, IHC-Fr

applications

免疫原

Native Full Length Protein corresponding to Human COL5A1.

P20908

特异性

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.В  This antibody reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p>Native conditions are recommended.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (<a href='/products/unavailable/collagen-v-antibody-biotin-ab6582'>ab6582</a>).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50.00000 - 1/200.00000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (<a href='/products/unavailable/collagen-v-antibody-biotin-ab6582'>ab6582</a>).</p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Can be used with a suitable biotinylated detection antibody such as Rabbit polyclonal to Collagen V (Biotin) (<a href='/products/unavailable/collagen-v-antibody-biotin-ab6582'>ab6582</a>).</p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
存储溶液
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Collagen V also known as collagen type V is a fibrillar collagen integral to the formation and structure of the extracellular matrix. It plays a mechanical role by regulating the assembly of other fibrillar collagens such as type I. Collagen V is a heterotrimer composed of three alpha chains—two α1(V) and one α2(V)—and demonstrates a molecular weight that typically ranges between 180-200 kDa. Expression of collagen type 5 is prominent in connective tissues especially those found in skin tendon and cornea where it ensures proper collagen fibril formation and tissue stability.
Biological function summary

Collagen V coordinates with other collagen types to control fibrillogenesis the process by which collagen molecules assemble into fibrils. It exists as part of a complex within the extracellular matrix often found interacting with collagen type I influencing its diameter and the organization of fibrils. This involvement is important for maintaining the structural integrity and mechanical properties of tissues that require a well-organized fibrillar network like the sclera and the dermis.

Pathways

Collagen V participates in the regulation of the collagen biosynthesis and degradation pathway as well as the TGF-Β signaling pathway. It modulates the biosynthesis pathway by ensuring a controlled assembly and deposition of collagen fibrils which is essential for tissue repair and maintenance. Through its influence on the TGF-Β signaling pathway collagen type V interacts with proteins such as decorin and biglycan which are also associated with modulating collagen fibril formation and tissue morphogenesis.

Collagen V defects or imbalances can lead to conditions such as Ehlers-Danlos syndrome (EDS) and fibrosis. EDS is associated with mutations in the COL5A1 or COL5A2 genes leading to abnormal collagen V production which affects skin elasticity and joint integrity. Fibrosis involves excessive collagen deposition including collagen type V resulting in tissue stiffening and impaired function. In these disorders surrounding collagens and matrix proteins like decorin may be altered further exacerbating tissue dysfunction.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
See full target information COL5A1
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文献 (56)

Recent publications for all applications. Explore the full list and refine your search

JID innovations : skin science from molecules to population health 3:100222 PubMed37789949

2023

M1/M2 Macrophage Skewing is Related to Reduction in Types I, V, and VI Collagens with Aging in Sun-Exposed Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Horiba,Munetaka Kawamoto,Ryozo Tobita,Ryota Kami,Yuki Ogura,Junichi Hosoi

International journal of molecular sciences 24: PubMed37176085

2023

Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Xu,Huabo Zheng,Pakhwan Nilcham,Octavian Bucur,Felix Vogt,Ioana Slabu,Elisa Anamaria Liehn,Mihaela Rusu

Skeletal muscle 13:1 PubMed36609344

2023

Multi-omics analysis of sarcospan overexpression in mdx skeletal muscle reveals compensatory remodeling of cytoskeleton-matrix interactions that promote mechanotransduction pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jackie L McCourt,Kristen M Stearns-Reider,Hafsa Mamsa,Pranav Kannan,Mohammad Hossein Afsharinia,Cynthia Shu,Elizabeth M Gibbs,Kara M Shin,Yerbol Z Kurmangaliyev,Lauren R Schmitt,Kirk C Hansen,Rachelle H Crosbie

Vascular biology (Bristol, England) 4:28-39 PubMed36222505

2022

Cell contractility and focal adhesion kinase control circumferential arterial stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Roberts,Tina Xu,Richard K Assoian

Hernia : the journal of hernias and abdominal wall surgery 26:1695-1705 PubMed36048398

2022

Similar collagen distribution in full-thickness skin grafts in intraperitoneal and onlay positions, an experimental mice-study.

Applications

Unspecified application

Species

Unspecified reactive species

A Winsnes,M-L Ivarsson,P Falk,U Gunnarsson,K Strigård

Cells 11: PubMed35563866

2022

Allogeneic Serum and Macromolecular Crowding Maintain Native Equine Tenocyte Function in Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Rampin,Ioannis Skoufos,Michael Raghunath,Athina Tzora,Nikolaos Diakakis,Nikitas Prassinos,Dimitrios I Zeugolis

British journal of pharmacology 179:2697-2712 PubMed34879432

2022

Transglutaminase 2: Development of therapeutic antibodies reveals four inhibitory epitopes and confirms extracellular function in fibrotic remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Mabrouka Maamra,Osama Mehdi Benayad,David Matthews,Catherine Kettleborough,John Atkinson,Katharine Cain,Helene Bon,Helen Brand,Michael Parkinson,Philip F Watson,Timothy S Johnson

Frontiers in oncology 12:813809 PubMed35280775

2022

COL5A2 Inhibits the TGF-β and Wnt/β-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Long Han,Dan Luo,Kakeng Habaxi,Julaiti Tayierjiang,Wei Zhao,Wei Wang,Wumaierjiang Aikebaier,Li Wang

NPJ breast cancer 8:11 PubMed35042889

2022

C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Britt A Sterken,Tobias Ackermann,Christine Müller,Hidde R Zuidhof,Gertrud Kortman,Alejandra Hernandez-Segura,Mathilde Broekhuis,Diana Spierings,Victor Guryev,Cornelis F Calkhoven

Advanced therapeutics 5:2100222 PubMed35310821

2022

Inhibition of Collagenase Q1 of as a Novel Antivirulence Strategy for the Treatment of Skin-Wound Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Alhayek,Essak S Khan,Esther Schönauer,Tobias Däinghaus,Roya Shafiei,Katrin Voos,Mitchell K L Han,Christian Ducho,Gernot Posselt,Silja Wessler,Hans Brandstetter,Jörg Haupenthal,Aránzazu Del Campo,Anna K H Hirsch
View all publications
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