Anti-Collagen IV抗体(ab6586)
Key features and details
- Rabbit polyclonal to Collagen IV
- Suitable for: ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFr
- Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey, Chinese hamster, Syrian hamster
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Collagen IV抗体
参阅全部 Collagen IV 一抗 -
描述
兔多克隆抗体to Collagen IV -
宿主
Rabbit -
特异性
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
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经测试应用
适用于: ELISA, IHC-Fr, WB, IHC-P, IP, ICC/IF, IHC-FrFl, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Zebrafish, African green monkey, Chinese hamster, Syrian hamster
预测可用于: Mammals
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免疫原
This product was produced with the following immunogens:
Full length native protein (purified) corresponding to Human Collagen IV. Collagen Type IV from human and bovine placenta. The immunogen maintains the native conformation of the protein.
Database link: P02462
Full length native protein (purified) corresponding to Bovine Collagen IV.
Database link: Q7SIB2 -
阳性对照
- IHC-P: Human kidney, liver, lung, renal oncocytoma and skeletal muscle tissues.
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常规说明
There are other recombinant monoclonal options, such as Recombinant Anti-Collagen IV antibody ab214417.
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Anti-Collagen Type IV has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. -
Primary antibody说明
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-Fr | (21) |
Use at an assay dependent concentration.
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| WB | (26) |
Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.
This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
| IHC-P | (36) |
1/15 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| IP | (3) |
Use at an assay dependent concentration.
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| ICC/IF | (23) |
Use at an assay dependent concentration. PubMed: 19933193
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| IF |
Use at an assay dependent concentration.
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| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| IHC-FoFr | (8) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 161 kDa. This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
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IHC-P
1/15 - 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 19933193 |
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IF
Use at an assay dependent concentration. |
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IHC-FrFl
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. |
靶标
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功能
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. -
组织特异性
Highly expressed in placenta. -
疾病相关
Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles. -
序列相似性
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
结构域
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
翻译后修饰
Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, arresten. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 282191 Cow
- Entrez Gene: 317711 Cow
- Entrez Gene: 407107 Cow
- Entrez Gene: 508632 Cow
- Entrez Gene: 511602 Cow
- Entrez Gene: 1282 Human
- Entrez Gene: 1284 Human
- Entrez Gene: 1285 Human
see all -
别名
- Arresten antibody
- BSVD antibody
- CO4A1_HUMAN antibody
see all
图片
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Collagen IV antibody (ab6586)This image is courtesy of an anonymous AbreviewImmunohistochemistry analysis of paraformaldehyde perfusion fixed frozen pig kidney permeabilized with 0.05% tween 20. Stained with ab6586 at 1/200 dilution. Secondary antibody used was Alexa Fluor® 647 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed at 1/300 dilution. The nuclei are stained Blue with Hoechst. The Red counter stain was created with a methyl green stain and by imaging with a 555nm excitation filter. Blocking was done with Sea Block for 30 minutes at 22°C.
The sample was incubated with the primary antibody and Sea Block for 16 hours at 4°C. Antigen retrieval method was heat mediated with Citrate Buffer pH 6.0. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)This image is courtesy of an anonymous AbreviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde fixed human brain (cortex) staining with ab6586 at 1/250 dilution. Secondary antibody used was HRP Goat Agilent anti-mouse/rabbit EnVision Dual Link.
Blocking was done using 5% serum for 15 minutes at 20°C. The sample was incubated with the primary antibody for 1 hour at 20°C with Agilent antibody diluent. Antigen retrieval method was heat mediated Citrate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Immunohistochemical analysis formalin-fixed paraffin-embedded human lung tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Immunohistochemical analysis of formalin-fixed paraffin-embedded human skeletal muscle cells labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers. Counterstained with hematoxylin for 15 seconds.
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Immunohistochemical analysis of formalin-fixed paraffin-embedded human renal oncocytoma tissue labelling Collagen IV with ab6586 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking: Peroxidase-Blocking Solution for 10 minutes. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests. Counterstained with hematoxylin for 15 seconds
Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Paraffin-embedded human kidney tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis with strong staining observed in glomeruli.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody (ab6586)Paraffin-embedded human liver tissue stained for Collagen IV using ab6586 at 1/400 dilution in immunohistochemical analysis, strong staining was observed in the sinusoids.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (920)
ab6586 被引用在 920 文献中.
- Umek N et al. Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice. Biomol Biomed 24:582-592 (2024). PubMed: 37902457
- Yang J et al. Bioactive decellularized adipose matrix prepared using a rapid, nonchemical/enzymatic method for adipogenesis. Biotechnol Bioeng 121:157-175 (2024). PubMed: 37691171
- Uçar MC et al. Self-organized and directed branching results in optimal coverage in developing dermal lymphatic networks. Nat Commun 14:5878 (2023). PubMed: 37735168
- Tora MS et al. Tumor microenvironment in a minipig model of spinal cord glioma. J Transl Med 21:667 (2023). PubMed: 37752585
- Salas A et al. Neovascular Progression and Retinal Dysfunction in the Laser-Induced Choroidal Neovascularization Mouse Model. Biomedicines 11:N/A (2023). PubMed: 37760886