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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen

Anti-Collagen III抗体[FH-7A] (ab6310)

  • Datasheet
  • SDS
Reviews (13)Q&A (24)References (162)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
  • Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

Key features and details

  • Mouse monoclonal [FH-7A] to Collagen III
  • Suitable for: IHC-Fr, IHC-P, WB, ELISA, Dot blot, Indirect ELISA, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Collagen III抗体[FH-7A]
    参阅全部 Collagen III 一抗
  • 描述

    小鼠单克隆抗体[FH-7A] to Collagen III
  • 宿主

    Mouse
  • 特异性

    ab6310 specifically recognizes collagen type III from human and rat origin. It does not recognize collagen types I, II, IV, V, VI and X.

  • 经测试应用

    适用于: IHC-Fr, IHC-P, WB, ELISA, Dot blot, Indirect ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照

    • IHC-P: Rat skin sections. IHC-Fr: Rat skin sections.
  • 常规说明

    Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

     

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.097% Sodium azide
    Constituent: Whole serum
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    FH-7A
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • Native Human Collagen III protein (ab7535)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6310于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P (10)
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
ELISA (2)
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 25136258
说明
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Dot blot
Use at an assay dependent concentration.
Indirect ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 25136258

靶标

  • 功能

    Collagen type III occurs in most soft connective tissues along with type I collagen.
  • 疾病相关

    Defects in COL3A1 are a cause of Ehlers-Danlos syndrome type 3 (EDS3) [MIM:130020]; also known as benign hypermobility syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS3 is a form of Ehlers-Danlos syndrome characterized by marked joint hyperextensibility without skeletal deformity.
    Defects in COL3A1 are the cause of Ehlers-Danlos syndrome type 4 (EDS4) [MIM:130050]. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS4 is the most severe form of the disease. It is characterized by the joint and dermal manifestations as in other forms of the syndrome, characteristic facial features (acrogeria) in most patients, and by proneness to spontaneous rupture of bowel and large arteries. The vascular complications may affect all anatomical areas.
    Defects in COL3A1 are a cause of susceptibility to aortic aneurysm abdominal (AAA) [MIM:100070]. AAA is a common multifactorial disorder characterized by permanent dilation of the abdominal aorta, usually due to degenerative changes in the aortic wall. Histologically, AAA is characterized by signs of chronic inflammation, destructive remodeling of the extracellular matrix, and depletion of vascular smooth muscle cells.
  • 序列相似性

    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • 翻译后修饰

    Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P02461 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1281 Human
    • Entrez Gene: 84032 Rat
    • Omim: 120180 Human
    • SwissProt: P02461 Human
    • SwissProt: P13941 Rat
    • Unigene: 443625 Human
    • Unigene: 3247 Rat
    • 别名

      • Alpha 1 type III collagen antibody
      • Alpha1 (III) collagen antibody
      • CO3A1_HUMAN antibody
      • COL 3A1 antibody
      • COL3A1 antibody
      • Collagen alpha 1(III) chain antibody
      • Collagen alpha-1(III) chain antibody
      • Collagen III alpha 1 chain precursor antibody
      • Collagen III alpha 1 polypeptide antibody
      • Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody
      • Collagen type III alpha 1 antibody
      • Collagen type III alpha 1 chain antibody
      • Collagen type III alpha antibody
      • Collagen, fetal antibody
      • EDS4A antibody
      • Ehlers Danlos syndrome type IV, autosomal dominant antibody
      • Fetal collagen antibody
      • Type III collagen antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

      Staining of formalin-fixed, paraffin-embedded rat skin with 1:4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (ab6310)This image is courtesy of an Abreview submitted by Birgitta Weijdegard

      ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120ºC in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

      This image was generated using the ascites version of the product.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)
      Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (ab6310)

      Staining of frozen rat skin sections with 1:8,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

      This image was generated using the ascites version of the product.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (162)

    发表研究结果有使用 ab6310?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6310 被引用在 162 文献中.

    • Ye JJ  et al. Phase-change composite filled natural nanotubes in hydrogel promote wound healing under photothermally triggered drug release. Bioact Mater 21:284-298 (2023). PubMed: 36157247
    • Malhotra R  et al. Matrix Gla Protein Levels Are Associated With Arterial Stiffness and Incident Heart Failure With Preserved Ejection Fraction. Arterioscler Thromb Vasc Biol 42:e61-e73 (2022). PubMed: 34809448
    • Shimozaki K  et al. Investigating the histological and structural properties of tendon gel as an artificial biomaterial using the film model method in rabbits. J Exp Orthop 9:1 (2022). PubMed: 34978637
    • Zhang Y  et al. Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nat Commun 13:2447 (2022). PubMed: 35508470
    • Chen J  et al. KRT17 Promotes the Activation of HSCs via EMT in Liver Fibrosis. J Clin Transl Hepatol 10:207-218 (2022). PubMed: 35528988
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 37 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
    Abreviews
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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 09 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
    Abreviews
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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 08 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (heart)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate, pH 6
    Permeabilization
    No
    Specification
    heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jul 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (femoral vein)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
    Permeabilization
    Yes - Triton X
    Specification
    femoral vein
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Aug 23 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
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    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    提交于 May 24 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 May 24 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    10% normal buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 May 24 2018

    ELISA abreview for Anti-Collagen III antibody [FH-7A]

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    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (purified protein and cell culture supernatant)
    Specification
    purified protein and cell culture supernatant
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Type
    Sandwich (Capture)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Patricia Carulla

    Verified customer

    提交于 Jun 28 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Collagen III antibody [FH-7A]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (First trimester human decidua)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 0.1% (W/V) Trypsin
    Permeabilization
    No
    Specification
    First trimester human decidua
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ruhul Choudhury

    Verified customer

    提交于 Feb 13 2017

    Question

    I'm the person who recently tested and submitted the feedback about the problem of non-validity of ab6310 on pig samples. I already got a replacement, so I guess I'm not untitled to any refund then, am I? Thanks a lot Best regards

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    Abcam community

    Verified customer

    Asked on Sep 04 2012

    Answer

    Thank you for contacting us. We offer either replacement or refund. As you have already received compensation in the form of a replacement antibody, you would be ineligible for a refund. Please note that your replacement product is covered by our Abpromise guarantee. We are happy provide scientific support at any time. If you are using the product in species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

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    Abcam Scientific Support

    回复于 Sep 04 2012

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