AB6310

Anti-Collagen III 抗体 [FH-7A]

Name: Collagen III抗体[FH-7A] (ab6310)| Abcam中文官网
Brand: Abcam Limited
SKU: AB6310
Price: 5235 CNY
Availability: InStock
Rating: 5 (14 reviews)

Anti-Collagen III antibody [FH-7A]

Lab Essentials
了解详情

(14 Reviews)

(194 Publications)

Anti-Collagen III antibody [FH-7A] (ab6310) is a mouse monoclonal antibody detecting Collagen III in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA, Dot Blot. Suitable for Human, Rat.

- Clone FH-7A is the most cited clone to Collagen III
- Over 170 publications
- Trusted since 2002

查看别名

Collagen alpha-1(III) chain, COL3A1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

Staining of formalin-fixed, paraffin-embedded rat skin with 1 : 4,000 ab6310 using biotin/ExtrAvidin®-Peroxidase.

This image was generated using the ascites version of the product.

Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

Staining of frozen rat skin sections with 1 : 8,000 ab6310 using biotin/ExtrAvidin^®-Peroxidase.

This image was generated using the ascites version of the product.

IHC-P

AbReview5837****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

ab6310 at 1/600 diltuion staining preovulatory follicle and whole ovary tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigens were retrieved by boiling with an antigen unmasking solution for 20 min at 120°C in an autoclave and then cooled down in water for 5 minutes. The tissue sections were formaldehyde fixed and incubated with the antibody for 1 hour. An alkaline phosphatase conjugated antibody was used as the secondary. The image shows a section of whole preovulatory follicle. Staining for collagen type III is seen in the theca interna cell layer. No staining in the granulosa cells.

This image was generated using the ascites version of the product.

This image is courtesy of an Abreview submitted by Birgitta Weijdegard

AbReview22934****

Western blot - Anti-Collagen III antibody [FH-7A] (AB6310)

The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.

PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.

All lanes:

Western blot - Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution

Lane 1:

plus <a href='/products/proteins-peptides/native-human-collagen-iii-protein-ab7535'>ab7535</a> reduced and denatured

Lane 2:

plus <a href='/products/proteins-peptides/native-human-collagen-iii-protein-ab7535'>ab7535</a> native

Secondary

All lanes:

Goat anti-mouse-HRP at 1/2000 dilution

false

Image courtesy of Michaela Leyh by Abreview.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

FH-7A

亚型

IgG1

不含载体蛋白

反应种属

Rat, Human

应用

ELISA, Dot, WB, ICC/IF, IHC-P, IHC-Fr

applications

免疫原

Native Full Length Protein corresponding to Human COL3A1.

P02461

特异性

ab6310 specifically recognizes collagen type III from human and rat origin. It does not recognize collagen types I, II, IV, V, VI and X.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

Anti-Collagen III antibody [FH-7A] (ab6310) is a House Mouse Monoclonal antibody and is validated for use in Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, WB in human samples.

Anti-Collagen III antibody [FH-7A] (ab6310) has been cited over 172 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-Collagen III antibody [FH-7A] (ab6310) has high sensitivity and specificity.

Anti-Collagen III antibody [FH-7A] (ab6310) has 13 independent reviews from customers.

Anti-Collagen III antibody [FH-7A] (ab6310) specifically detects Collagen III (UniProt ID: P02461; Molecular weight: 95kDa) and is sold in 50 uL selling sizes.

Collagen III is a critical fibrillar collagen that plays a significant role in the structural integrity of various tissues, including the skin, lungs, and blood vessels. Alterations in Collagen III expression are associated with several diseases. For instance, mutations in the COL3A1 gene, which encodes Collagen III, can lead to Ehlers-Danlos syndrome type IV, a condition characterized by fragile blood vessels and organs. Additionally, abnormal Collagen III deposition is implicated in fibrotic diseases, such as liver cirrhosis and pulmonary fibrosis, where excessive collagen accumulation disrupts normal tissue architecture. Understanding Collagen III's role in these diseases is crucial for developing targeted therapies and improving patient outcomes.

Type III collagen, [a1(III)]3 ,is an approx. 300 kDa molecule, found predominantly in skin, blood vessels, liver, placenta, tongue, and thymus. Collagen type III forms cofibrils with type I and/or V collagens in a number of tissues of mesenchymal origin, such as skin, tendon, ligaments, and bone. This collagen type is involved, directly or indirectly in several genetic diseases, including Ehlers-Danlos type IV disease.

This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

style

left-column

性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.097% Sodium azide Constituents: Whole serum

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.

Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

See full target information COL3A1

style

left-column

文献 (194)

Recent publications for all applications. Explore the full list and refine your search

Hepatology (Baltimore, Md.) : PubMed41370247

2025

Spatiotemporal landscape of intrahepatic cholangiocarcinoma liver metastasis at the single-cell level.

Applications

mIHC

Species

Human

Ziyu Xun,Hao Wang,Zhengfeng Xuan,Kexu Xiong,Nan Zhang,Junyu Long,Huishan Sun,Yiran Li,Chengpei Zhu,Mingjian Piao,Ting Zhang,Longhao Zhang,Shuofeng Li,Chengjie Li,Jiongyuan Li,Boyu Sun,Zixiang Zhou,Shanshan Wang,Ziyue Huang,Kai Liu,Yang Tan,Xiaohua Shi,Xiaobo Yang,Hanping Wang,Ling Lu,Haitao Zhao

JCI insight 10: PubMed40627450

2025

CAR-engineered cytolytic Tregs reverse pulmonary fibrosis and remodel the fibrotic niche with limited CRS.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Han Jiang,Meng Zhou,Meng-Di Cheng,Sai Chen,Ying-Qiang Guo

Scientific reports 15:20528 PubMed40594269

2025

Characterization of collagen profile in peritoneal metastases of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

P Villarejo-Campos,S García Gómez-Heras,S González-Moreno,S Qian Zhang,R Franco-Rodríguez,I Díaz-Caro,R Olivera-Salazar,D García-Olmo,M García-Arranz

Scientific reports 15:20025 PubMed40481016

2025

Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Zhou,Madalena Pinto Gomes,Anouk Elgersma,H Ibrahim Korkmaz,Bouke K H L Boekema,Marie Louise Groot

Stem cell research & therapy 15:462 PubMed39627883

2024

Exosomes derived from minor salivary gland mesenchymal stem cells: a promising novel exosome exhibiting pro-angiogenic and wound healing effects similar to those of adipose-derived stem cell exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Xiang,Pengbing Ding,Jiaying Qian,Enhang Lu,Yimou Sun,Seyeon Lee,Zhenkun Zhao,Zhixuan Sun,Zhenmin Zhao

Biomaterials translational 5:157-174 PubMed39351163

2024

Electrical stimulation with polypyrrole-coated polycaprolactone/silk fibroin scaffold promotes sacral nerve regeneration by modulating macrophage polarisation.

Applications

Unspecified application

Species

Unspecified reactive species

Haofeng Cheng,Jun Bai,Xingyu Zhou,Nantian Chen,Qingyu Jiang,Zhiqi Ren,Xiangling Li,Tianqi Su,Lijing Liang,Wenli Jiang,Yu Wang,Jiang Peng,Aijia Shang

PloS one 19:e0301216 PubMed38743641

2024

In vivo study on the repair of rat Achilles tendon injury treated with non-thermal atmospheric-pressure helium microplasma jet.

Applications

Unspecified application

Species

Unspecified reactive species

Katusmasa Nakazawa,Hiromitsu Toyoda,Tomoya Manaka,Kumi Orita,Yoshihiro Hirakawa,Kosuke Saito,Ryosuke Iio,Akiyoshi Shimatani,Yoshitaka Ban,Hana Yao,Ryosuke Otsuki,Yamato Torii,Jun-Seok Oh,Tatsuru Shirafuji,Hiroaki Nakamura

Journal of anatomy 245:217-230 PubMed38624036

2024

Investigating the role connective tissue fibroblasts play in the altered muscle anatomy associated with the limb abnormality, Radial Dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

George R F Murphy,Eleanor Feneck,James Paget,Branavan Sivakumar,Gill Smith,Malcolm P O Logan

Histochemistry and cell biology 161:367-379 PubMed38347221

2024

Fibrosis and expression of extracellular matrix proteins in human interventricular septum in aortic valve stenosis and regurgitation.

Applications

Unspecified application

Species

Unspecified reactive species

David Sedmera,Alena Kvasilova,Adam Eckhardt,Petr Kacer,Martin Penicka,Matej Kocka,Dana Schindler,Ron Kaban,Radka Kockova

Kidney & blood pressure research 48:611-627 PubMed37717559

2023

Transcription Factor Sox9 Exacerbates Kidney Injury through Inhibition of MicroRNA-96-5p and Activation of the Trib3/IL-6 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Guang Chen,Yongqiang Du,Jiajia Yang,Wei Wang

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen III 抗体 [FH-7A]

Anti-Collagen III antibody [FH-7A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

Immunohistochemistry (Frozen sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

Western blot - Anti-Collagen III antibody [FH-7A] (AB6310)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性