AB224532

重组Anti-Collagen III抗体[EPR17673] - BSA and Azide free

Anti-Collagen III antibody [EPR17673] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Collagen III antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

Collagen alpha-1(III) chain, COL3A1

19 Images

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Collagen III with ab184993 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993). Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : HeLa whole cell lysate 10 µg (Input). Lane 2 : ab184993 IP in HeLa whole cell lysate . Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate . Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>)

Lane 1:

HeLa whole cell lysate 10 µg (Input)

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5.5s

Supplier Data

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab184993 IP in HeLa whole cell lysate .

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate .

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>)

Predicted band size: 139 kDa

Observed band size: 150 kDa

false

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using ab184993, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green).Confocal image showing cytoplasmic staining in PC-12 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control : Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using ab184993, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control : Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993). Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993). Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This image was produced using ab237239, the same clone but in a different formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cells) labelling Collagen III with ab237239 at 1/50 (10.0 μg/ml). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 488) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in PC-12 cell line and no staining in rat splenocytes (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Rat splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This image was produced using ab237238, the same clone but in a different formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) labelling Collagen III with ab237238 at 1/50 (10.0 μg/ml). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in PC-12 cell line and no staining in rat splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Rat splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This image was produced using ab237238, the same clone but in a different formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophages) labelling Collagen III with ab237238 at 1/50 (10.0 μg/ml). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in RAW 264.7 cell line and no staining in mouse splenocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Mouse splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This image was produced using ab237239, the same clone but in a different formulation.

Immunofluorescent analysis of 4% Paraformaldehyde fixed 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophages) labelling Collagen III with ab237239 at 1/50 (10.0 μg/ml). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 488) was used as a counterstain at 1/200 dilution (2.5μg/ml). Confocal image showing cytoplasmic staining in RAW 264.7 cell line and no staining in mouse splenocytes (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).

Negative control : Mouse splenocytes.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993). Collagen III was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : RAW 264.7 whole cell lysate 10 μg (Input). Lane 2 : ab184993 IP in RAW 264.7 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in RAW 264.7 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3.25 seconds. Lysate was freshly made and used immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>)

Lane 1:

RAW 264.7 whole cell lysate 10 μg (Input).

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3.25s

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993). Collagen III was immunoprecipitated from 0.35 mg of PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : PC-12 whole cell lysate 10 μg (Input). Lane 2 : ab184993 IP in PC-12 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in PC-12 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3.25 seconds. Lysate was freshly made and used immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysate 10 μg (Input).

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using ab184993, the same antibody clone in a different buffer formulation. Exposure time : Lanes 1-3 : 30 seconds; Lane 4 : 5 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26017148).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>) at 1/1000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 2 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Supplier Data

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using ab184993, the same antibody clone in a different buffer formulation. Exposure time : Lane 1 : 3 minutes; Lane 2 : 15 seconds; Lane 3 : 3 seconds; Lanes 4-5 : 30 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26017148).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 5:

HaCaT (human keratinocyte cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Lab

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

This data was developed using ab184993, the same antibody clone in a different buffer formulation. Exposure time : Lanes 1-4 : 92 seconds; Lanes 5-6 : 48 seconds; Lane 7 : 37 seconds. Blocking/Dilution buffer : 5% NFDM/TBST. In lane 1-4, the lysates were stored at -80℃ prior to Western Blotting. In lane 5-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell fresh lysate at 20 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell fresh lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma), whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

false

Lab

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184993).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (<a href='/products/primary-antibodies/collagen-iii-antibody-epr17673-ab184993'>ab184993</a>) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Exposure time: 3min

不同偶联物与剂型 (3)

Unconjugated

Anti-Collagen III antibody [EPR17673]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Collagen III antibody [EPR17673]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Collagen III antibody [EPR17673]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17673

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

IP, Flow Cyt (Intra), WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab224532 is the carrier-free version of ab184993.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.

Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

See full target information COL3A1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature aging 1:698-714 PubMed34746803

2021

Senescent cells suppress innate smooth muscle cell repair functions in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bennett G Childs,Cheng Zhang,Fahad Shuja,Ines Sturmlechner,Shawn Trewartha,Raul Fierro Velasco,Darren Baker,Hu Li,Jan M van Deursen

View all publications

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重组Anti-Collagen III抗体[EPR17673] - BSA and Azide free

Anti-Collagen III antibody [EPR17673] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

Western blot - Anti-Collagen III antibody [EPR17673] - BSA and Azide free (AB224532)

不同偶联物与剂型 (3)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (1)

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搭配使用产品

Assay Kits

Reagents, Controls & Accessories

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Secondary Antibodies

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Primary Antibodies

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