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AB184993

重组Anti-Collagen III抗体[EPR17673]

Anti-Collagen III antibody [EPR17673]

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(158 Publications)

Anti-Collagen III antibody [EPR17673] (ab184993) is a rabbit monoclonal antibody detecting Collagen III in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 70 publications

查看别名

Collagen alpha-1(III) chain, COL3A1

15 Images
Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on MCF7 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Collagen IIIwith ab184993 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Collagen III with ab184993 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)
  • IP

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)

Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : HeLa whole cell lysate 10 µg (Input).  Lane 2 : ab184993 IP in HeLa whole cell lysate .  Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate . Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 5.5 seconds.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

Lane 1:

HeLa whole cell lysate 10 µg (Input)

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 5.5s

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)
  • IP

Supplier Data

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)

Collagen III was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (Input).

Lane 2 : ab184993 IP in HeLa whole cell lysate .

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in HeLa whole cell lysate .

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

Predicted band size: 139 kDa

Observed band size: 150 kDa

false

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma)  cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma small irregularly shaped cells) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green).Confocal image showing cytoplasmic staining in PC-12 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control : Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Collagen III antibody [EPR17673] (AB184993)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution. Secondary antibody only control : Used PBS instead of primary antibody, secondary is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody (ab150081) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Collagen III antibody [EPR17673] (AB184993)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell line labeling Collagen III with ab184993 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)
  • IP

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)

Collagen III was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : RAW 264.7 whole cell lysate 10 μg (Input).  Lane 2 : ab184993 IP in RAW 264.7 whole cell lysate.  Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in RAW 264.7 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3.25 seconds. Lysate was freshly made and used immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993)

Lane 1:

RAW 264.7 whole cell lysate 10 μg (Input).

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3.25s

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)
  • IP

Lab

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (AB184993)

Collagen III was immunoprecipitated from 0.35 mg of PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate with ab184993 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184993 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution. Lane 1 : PC-12 whole cell lysate 10 μg (Input).  Lane 2 : ab184993 IP in PC-12 whole cell lysate.  Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab184993 in PC-12 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3.25 seconds. Lysate was freshly made and used immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysate 10 μg (Input).

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell), whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3s

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)
  • WB

Supplier Data

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)

Exposure time : Lanes 1-3 : 30 seconds; Lane 4 : 5 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26017148).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

Lane 1:

Human skin lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Lane 4:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 10 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 2 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)
  • WB

Supplier Data

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 15 seconds; Lane 3 : 3 seconds; Lanes 4-5 : 30 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 26017148).

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human fetal kidney lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 5:

HaCaT (human keratinocyte cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)
  • WB

Lab

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)

Exposure time : Lanes 1-4 : 92 seconds; Lanes 5-6 : 48 seconds; Lane 7 : 37 seconds. Blocking/Dilution buffer : 5% NFDM/TBST. In lane 1-4, the lysates were stored at -80℃ prior to Western Blotting. In lane 5-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell fresh lysate at 20 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell fresh lysate at 20 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma), whole cell fresh lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

false

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)
  • WB

Lab

Western blot - Anti-Collagen III antibody [EPR17673] (AB184993)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Collagen III antibody [EPR17673] (ab184993) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 150 kDa

true

Exposure time: 3min

不同偶联物与剂型 (3)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17673

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

ICC/IF, Flow Cyt (Intra), IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

Anti-Collagen III antibody [EPR17673] (ab184993) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IP, WB in human, mouse, rat samples.

Anti-Collagen III antibody [EPR17673] (ab184993) has been cited over 70 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Collagen III antibody [EPR17673] (ab184993) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Collagen III antibody [EPR17673] (ab184993) specifically detects Collagen III (UniProt ID: P02461; Molecular weight: 95kDa) and is sold in 100 uL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR17673 - ab224532.

Antibody clone EPR17673 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (ab237238, ab237239).

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type III also known as COL3 and collagen III is a fibrillar protein with an approximate molecular mass of 138 kDa. This protein is a significant component of the extracellular matrix and is mainly expressed in connective tissues like skin lungs and vascular walls. You can also find it in developing tissues and fibrotic tissues reflecting its role during tissue growth and repair processes.
Biological function summary

Collagen type III contributes to maintaining the structural integrity of tissues. It forms a complex with collagen type I providing a scaffold that ensures mechanical strength and stability to tissues. This structural pairing of collagen type I and III highlights their cooperative function in supporting the extracellular matrix and regulating cell behavior in tissue repair and wound healing processes.

Pathways

Collagen type III is integral to the processes of tissue repair and fibrosis pathways. It interacts closely with proteins such as fibronectin and integrins contributing to the cellular adhesion and signaling pathways important for fibroblast function and migration. These interactions reflect its essential role in matrix assembly and stability influencing how cells respond to injury and repair damaged tissues.

Collagen type III mutations or dysregulation associate with vascular Ehlers-Danlos syndrome and keloids. In vascular Ehlers-Danlos syndrome defective collagen III compromises vessel wall integrity resulting in vascular fragility and rupture. In the context of keloids excessive collagen III accumulation in the skin illustrates its role in pathological fibrosis. Collagen type I is another protein that plays an important role in these conditions contributing along with collagen type III to the altered structural and functional properties observed in these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.
See full target information COL3A1
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文献 (158)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40999952

2025

HSPD1 regulates angiotensin II‑induced atrial fibrillation via the PPAR signaling pathway.

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Species

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Yimeng Zhou,Lianzhi Zhang,Shunping Zhou,Wenjia Zhang,Qunlin Gong,Nan Xu,Jiahong Wang,Zhong Zhang,Nannan Chen

PloS one 20:e0332892 PubMed40966241

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Inhibition of LncRNA Kcnq1ot1 suppresses hypoxia-induced pyroptosis of H9C2 cells by regulating miR-27b-3p.

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Species

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Yingjie Yang,Yanchun Ou,Guanlian Mo,Jing Wen,Limin Liang,Shirong Wang,Jinyi Li

Bioactive materials 54:159-178 PubMed40895235

2025

Injectable sustained-release danshensu sodium-loaded nanoparticle hydrogel targets macrophages to improve myocardial microenvironment for myocardial infarction treatment.

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Jianqiu Yang,Jing Wang,Zhihua Zeng,Zhenyu Chen,Dingguo Wang,Yongbing Wu

Biologics : targets & therapy 19:497-510 PubMed40893173

2025

Therapeutic Potential of Compounds with High Affinity to BAG2 in Inhibiting Keloid Disease.

Applications

Unspecified application

Species

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Yinmin Wang,Zhaoqi Yuan,Renpeng Zhou,Lin Lu,Xiuxia Wang,Jun Yang

ACS omega 10:36994-37003 PubMed40893218

2025

Potential Antiphotoaging Effect of Human Cathelicidin LL-37 Fragments and KR-12 Analogs on UVB-Induced HaCaT Cells and UVA-Induced HDF Cells.

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Species

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Menggeng Li,Jing Wang,Peng Shu,Xueqing Chen,Yizhen Yan,Jiangming Zhong,Nan Zhao,Ling Liang,Zhao Liu

Regenerative biomaterials 12:rbaf062 PubMed40708704

2025

Two-in-one: multifunctional poloxamer hydrogel accelerates endometrial regeneration and fertility restoration through synergistic regulation of KGF-2 and NO.

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Species

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Yijia Zhang,Xinji Wang,Qin Gu,Cuitao Lu,Yingzheng Zhao,Xiaokun Li

iScience 28:113013 PubMed40686602

2025

Cardiac fibrosis inhibitor CTPR390 prevents structural and morphological changes in human engineered cardiac connective tissue.

Applications

Unspecified application

Species

Unspecified reactive species

David Maestro,Ana Palanca,Helena Soto,I Llarena,Alisa Nicole DeGrave,Gabriela Guedes,Guilherme Henrique de Oliveira,André Luiz Coelho Conceição,Verónica Mieites,Jose M Icardo,Carlos Sanchez-Cano,Olga M Conde,Susanne Lutz,Aitziber L Cortajarena,Ana V Villar

Frontiers in pharmacology 16:1502269 PubMed40642013

2025

Hydrogen sulfide alleviates high-salt-stimulated myocardial fibrosis through inhibiting hypoxia-inducible factor-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Peng,Pan Huang,Boyang Lv,Chaoshu Tang,Hongfang Jin,Yaqian Huang

Frontiers in immunology 16:1538046 PubMed40547034

2025

Wharton's jelly-derived mesenchymal stem cells ameliorate high altitude-induced heart injury by promoting type 2 macrophage polarization via COX2-PGE2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhong Zhang,Li Zhao,Jing Cui,Yan Zhang,Dongtao Li,Zhibo Hong,Jiamin Liu,Shan Wang,Ningkun Zhang,Yang Li,Yu Chen

Journal of cell communication and signaling 19:e70025 PubMed40520075

2025

Adipose-derived stem cell exosomes alleviate TGF-β1-induced urethral stricture fibrosis by suppressing the TGF-β/Smad pathway and downstream PDGFR-β/RAS/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liang,Chao Deng,Hang Guo,Zhenghao Dai,Yiwen Jiang,Yuting Lu,Weiguo Chen
View all publications
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Primary Antibodies

AB6310

Anti-Collagen III antibody [FH-7A]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody [FH-7A] (AB6310)

  • 5
  • 14
Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

  • 4
  • 24

搭配使用产品

Primary Antibodies

AB316222

Anti-Collagen I antibody [RM1131]

BOND RX™ Validated|Recombinant|RabMAb|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] (AB316222)

  • 0
  • 0
Assay Kits

AB222941

Hydroxyproline Assay Kit (Colorimetric)

Biochemical assay - Hydroxyproline Assay Kit (Colorimetric) (AB222941)

  • 3
  • 4
Primary Antibodies

AB34710

Anti-Collagen I + Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

  • 5
  • 91
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB260043

Anti-Collagen I antibody [EPR22894-89]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043)

  • 4
  • 3

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