Anti-Collagen III 抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

Immunohistochemistry of ab7778. Tissue : right lobe of the liver section. A : Central Vein (CV) fibrosis, B : Non-fibrotic CV, C : Perisinusodial fibrosis, D : Non-fibrotic area, E : Protat tract fibrosis, F : Septal fibrosis (arrow). Fixation : formalin fixed paraffin embedded. Antigen retrieval : not required. Primary antibody : Anti-collagen type I at 1 : 500 for 4°C for 24hr. Secondary antibody : Peroxidase biotin-streptavidin rabbit secondary antibody at 1 : 10,000 for 45 min at RT. Localization : Anti-collagen type III is intra and extracellular. Staining : 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

ab7778 at 1 : 400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 (ab64214) at 99-100°C - 20 minutes for antigen retrieval.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

ab53088 staining Human skin (ab30166). Staining is localised to the extracellular matrix.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• IHC-P

AbReview25831****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

Image courtesy of Rudolf Jung by Abreview.

• WB

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Western blot - Anti-Collagen III antibody (AB7778)

Western Blot produced under denaturing and reducing conditions.

All lanes:

Western blot - Anti-Collagen III antibody (ab7778) at 1/1000 dilution

All lanes:

Human collagen III at 0.1 µg

Predicted band size: 139 kDa

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• WB

CiteAb

Western blot - Anti-Collagen III antibody (AB7778)

Western Blotting using Anti-Collagen III antibody, ab7778. Publication image from Xu, X. H. et al., 2019, Exp Mol Med, 30804321. Legend direct from paper.

Collagen production was induced in LOX- or LOXL2-downregulated preeclamptic placenta and trophoblast cells.a Representative hematoxylin-eosin staining and Masson’s trichrome staining of placenta and villi are shown. b Amount of total collagen was determined by measuring the hydroxyproline contents of placental tissues. c Western blot analysis of type I collagen, type III collagen, and type IV collagen in the placenta. d Statistical analysis of protein densitometry quantification of western blot analysis (c) by Student’s t-test. Data are presented as the means ± SEM. e Amount of soluble collagen from cell culture supernatants was quantified using a sircol collagen assay. Data are presented as the means ± SEM of three independent experiments. f Quantification of COL1A1, COL3A1, and COL4A1 mRNA expression levels determined in HTR-8/SVneo cells after siRNA treatment. Data are presented as the means ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ***P < 0.001; and NS not significant

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• WB

CiteAb

Western blot - Anti-Collagen III antibody (AB7778)

Western Blotting using Anti-Collagen I antibody, ab7778. Publication image from Yao, H. et al., 2015, Nat Commun, 26436920. Legend direct from paper.

miR-142-5p and miR-130a-3p regulate the profibrogenesis of macrophages.(a–g) Human macrophages were transduced with control, miR-142-5p ASO or miR-130a-3p mimics or both. After 24 h, the macrophages were stimulated with IL-4 for 12 h and co-cultured with primary human fibroblasts for another 48 h. (a) The schematics of the approach. (b) Representative images of fluorescent FAP/DAPI staining in fibroblasts. Scale bar, 100µm. Fluorescence intensity was quantitated.(mean±s.e.m., n=3 independent experiments; *P<0.05; **P<0.01. P values were obtained using two-tailed Student's t-test). (c) Fibroblast contractility in three-dimensional collagen matrices (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01. P values were obtained using two-tailed Student's t-test). (d) Representative images of western blot analysis ofα-SMA, collagen I and collagen III in fibroblasts (n=3). The numbers above the blots present the intensity ratio of indicated protein/GAPDH analysed by ImageJ. (e) Extracellular acid-soluble collagen production of fibroblasts was measured by the Sircol assay. (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01; ***P<0.001. P values were obtained using two-tailed Student's t-test). (f) The proliferation of fibroblasts was determined by BrdU incorporation assay (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01. P values were obtained using two-tailed Student's t-test). (g) Total TGF-β1 (acid-treated) and active TGF-β1 (not acid-treated) in the media of the macrophage/fibroblast co-culture system (mean±s.e.m., n=4 independent experiments; *P<0.05; **P<0.01; ***P<0.001. P values were obtained using two-tailed Student's t-test).

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