AB317539

Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907]

Name: Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (ab317539) | Abcam中文官网
Brand: Abcam Limited
SKU: AB317539

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Rabbit Multiclonal COL1A1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1.

查看别名

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded human breast carcinoma. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse testis. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded rat kidney. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Supplier Data

Western blot - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317539 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (ab317539) at 1/2000 dilution

Lane 1:

U87-MG whole cell extract at 30 µg

Lane 2:

U87-MG conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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关键信息

宿主种属

Rabbit

克隆

Multiclonal

克隆号

HL2048 + HL1907

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse, Rat

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human COL1A1.

P02452

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Collagen I + II + III are major components of the extracellular matrix providing structural support to various tissues. They are sometimes referred to as fibrillar collagens due to their ability to form strong fibrillar structures. Each type consists of three polypeptide chains wound into a triple helix with type I collagen having a molecular mass around 300 kDa. Collagen I is mostly found in skin tendons and bones while collagen II is abundant in cartilage. Collagen III is primarily located in skin blood vessels and internal organs. Together they provide tensile strength and are key to tissue integrity.

Biological function summary

Collagen I + II + III support tissue resilience and elasticity enabling organs and structures to endure stress while maintaining shape. They are integral to the formation of extracellular matrix complexes and interact with other matrix proteins like elastin and fibronectin. Through these interactions they not only provide structural support but also influence cell behavior affecting processes like cell proliferation and differentiation. These collagens contribute to wound healing by forming a scaffold that facilitates cell migration and tissue remodeling.

Pathways

These collagens influence the signaling pathways involved in tissue homeostasis and repair. They are part of the transforming growth factor-beta (TGF-β) signaling pathway that manages cell growth and differentiation. Collagen binding to integrins also affects the PI3K/Akt pathway which regulates cell survival and metabolism. These interactions highlight their role in maintaining the extracellular matrix's integrity with relationships to other extracellular matrix proteins like laminins and integrins.

Abnormalities in collagen I + II + III are linked to connective tissue disorders such as osteogenesis imperfecta and Ehlers-Danlos syndrome. Osteogenesis imperfecta related to collagen I mutations results in brittle bone pathology due to impaired collagen fibril formation. In contrast mutations in collagen II are associated with cartilage disorders like Stickler syndrome affecting vision and joint function. These conditions highlight the significance of collagenous proteins in tissue stability and functionality interfacing with proteins involved in inflammatory responses and tissue repair.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

其他靶点

COL3A1,COL2A1

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Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907]