Anti-Collagen I抗体[COL-1] (ab6308)
Key features and details
- Mouse monoclonal [COL-1] to Collagen I
- Suitable for: IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Collagen I抗体[COL-1]
参阅全部 Collagen I 一抗 -
描述
小鼠单克隆抗体[COL-1] to Collagen I -
宿主
Mouse -
经测试应用
适用于: IHC-Fr, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Cow, Pig, Deer -
免疫原
Full length native protein (purified) corresponding to Cow Collagen I. Cow skin collagen type I
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表位
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P. -
阳性对照
- WB: Natural Cow Collagen I protein (ab7526), IHC-Fr: Human tonsil tissue.
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常规说明
Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.0976% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from Tissue culture supernatant. -
克隆
单克隆 -
克隆编号
COL-1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
应用 | Ab评论 | 说明 |
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IHC-Fr | (2) |
Use a concentration of 3.5 - 7 µg/ml.
(amplification required).Use on unfixed tissue or acetone fixed tissue. |
WB | (2) |
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions. Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
说明 |
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IHC-Fr
Use a concentration of 3.5 - 7 µg/ml. (amplification required).Use on unfixed tissue or acetone fixed tissue. |
WB
Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions. Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
靶标
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功能
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
组织特异性
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
疾病相关
Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF. -
序列相似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain. -
翻译后修饰
Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 282187 Cow
- Entrez Gene: 282188 Cow
- Entrez Gene: 1277 Human
- Entrez Gene: 1278 Human
- Entrez Gene: 29393 Rat
- Entrez Gene: 84352 Rat
- Omim: 120150 Human
- Omim: 120160 Human
see all -
别名
- Alpha 1 type I collagen antibody
- Alpha 2 type I collagen antibody
- alpha 2 type I procollagen antibody
see all
图片
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Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi
Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.
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Lane 1 : Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/ml
Lane 2 : Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/ml
Lane 3 : Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/ml
Lane 4 : Anti-Collagen I antibody [COL-1] (ab6308) at 0 µg/ml
All lanes : Recombinant human Collagen 1
Predicted band size: 130 kDa -
Western blot - Anti-Collagen I antibody [COL-1] (ab6308)This image is courtesy of an anonymous AbreviewAnti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg
Secondary
An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 130 kDa
Exposure time: 24 hours
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Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (434)
ab6308 被引用在 434 文献中.
- Lu K et al. N-Acetyl-L-cysteine facilitates tendon repair and promotes the tenogenic differentiation of tendon stem/progenitor cells by enhancing the integrin α5/β1/PI3K/AKT signaling. BMC Mol Cell Biol 24:1 (2023). PubMed: 36604630
- He Y et al. Fabrication of a bio-instructive scaffold conferred with a favorable microenvironment allowing for superior implant osseointegration and accelerated in situ vascularized bone regeneration via type H vessel formation. Bioact Mater 9:491-507 (2022). PubMed: 34820585
- Li C et al. Rho kinase inhibition ameliorates vascular remodeling and blood pressure elevations in a rat model of apatinib-induced hypertension. J Hypertens 40:675-684 (2022). PubMed: 34862331
- Sun K et al. Osteogenesis and angiogenesis of a bulk metallic glass for biomedical implants. Bioact Mater 8:253-266 (2022). PubMed: 34541400
- Zhu X et al. Fabrication of Size-Controllable and Arrangement-Orderly HepG2 Spheroids for Drug Screening via Decellularized Liver Matrix-Derived Micropattern Array Chips. ACS Omega 7:2364-2376 (2022). PubMed: 35071924