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重组RabMAb

重组Anti-Colec12抗体[EPR29371-589] - BSA and Azide free (ab317631)

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Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
  • Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR29371-589] to Colec12 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Colec12抗体[EPR29371-589] - BSA and Azide free
    参阅全部 Colec12 一抗

  • 描述

    兔单克隆抗体[EPR29371-589] to Colec12 - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    Unsuitable for rat IHC

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC/IF or IP

  • 种属反应性

    与反应: Rat, Human
    不与反应: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Untreated 293T, 293T treated with Peptide:N-glycosidase F whole cell, LNCaP whole cell, HUVEC whole cell, Human placenta tissue, Human lung tissue, Human pancreas tissue, Rat lung tissue, Rat placenta tissue lysates. IHC-P: Human placenta and Human lung tissues.

  • 常规说明

    ab317631 is the carrier-free version of ab317630

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab317631于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 82 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.

靶标

  • 功能

    Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid beta in Alzheimer disease.

  • 组织特异性

    Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed in placenta. Moderately expressed in heart, skeletal muscle, small intestine and lung. Weakly expressed in brain, colon, thymus and kidney. Expressed in nurse-like cells. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of Alzheimer patient.

  • 序列相似性

    Contains 1 C-type lectin domain.
    Contains 3 collagen-like domains.

  • 细胞定位

    Membrane. Forms clusters on the cell surface.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CL P1 antibody
      • CL-P1 antibody
      • CLP1 antibody
      • COL12_HUMAN antibody
      • COLEC 12 antibody
      • colec12 antibody
      • Collectin placenta 1 antibody
      • Collectin placenta protein 1 antibody
      • Collectin sub family member 12 antibody
      • Collectin-12 antibody
      • hCL P1 antibody
      • hCL-P1 antibody
      • NSR2 antibody
      • Nurse cell scavenger receptor 2 antibody
      • SCARA4 antibody
      • Scavenger receptor class A member 4 antibody
      • Scavenger receptor with C type lectin antibody
      • Scavenger receptor with C-type lectin antibody
      • SRCL antibody
      see all

    图片

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      All lanes : Anti-Colec12 antibody [EPR29371-589] (ab317630) at 1/1000 dilution

      Lane 1 : Untreated 293T (human embryonic kidney epithelial cell) whole cell lysate
      Lane 2 : 293T cell lysate treated with Peptide:N-glycosidase F (PNGase F)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 82 kDa
      Observed band size: 110-150,90 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Collectin-12 is a glycoprotein of approximately 110-150 kDa and detected as a 90-kDa band after treated with Peptide:N-glycosidase F (PNGase F). 

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      All lanes : Anti-Colec12 antibody [EPR29371-589] (ab317630) at 1/1000 dilution

      Lane 1 : LNCaP (human prostate carcinoma epithelial cell) whole cell lysate
      Lane 2 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
      Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
      Lane 4 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 82 kDa
      Observed band size: 110,130 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: A549, Jurkat (PMID:12761161).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      All lanes : Anti-Colec12 antibody [EPR29371-589] (ab317630) at 1/1000 dilution

      Lane 1 : Human placenta tissue lysate
      Lane 2 : Human lung tissue lysate
      Lane 3 : Human liver tissue lysate
      Lane 4 : Human pancreas tissue lysate
      Lane 5 : Rat lung tissue lysate
      Lane 6 : Rat placenta tissue lysate
      Lane 7 : Rat liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-4 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
      Lanes 5-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 82 kDa
      Observed band size: 110,130,150 kDa why is the actual band size different from the predicted?



      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Low expression: liver (PMID: 11564734, 16868960), pancreas (PMID:11564734).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (16868960, 11564734).

      Lanes 5-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 and lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 26 seconds, lane 6: 37 seconds, lanes 2-5 and 7: 180 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Colec12 with ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human placenta (PMID: 18423602)
      The section was incubated with ab317630 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Colec12 with ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on the macrophages of human lung (PMID: 18423602)
      The section was incubated with ab317630 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Colec12 with ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression: no staining on human liver.
      The section was incubated with ab317630 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)
      Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

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