Anti-COL4A2抗体(ab125208)
Key features and details
- Rabbit polyclonal to COL4A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-COL4A2抗体
参阅全部 COL4A2 一抗 -
描述
兔多克隆抗体to COL4A2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human COL4A2 (C terminal).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125208于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 168 kDa.
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| IHC-P |
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 168 kDa. |
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IHC-P
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. -
序列相似性
Belongs to the type IV collagen family.
Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain. -
结构域
Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain. -
翻译后修饰
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
Proteolytic processing produces the C-terminal NC1 peptide, canstatin. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1284 Human
- Omim: 120090 Human
- SwissProt: P08572 Human
- Unigene: 508716 Human
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别名
- Canstatin antibody
- CO4A2_HUMAN antibody
- COL4A 2 antibody
see all
图片
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Western blot - Anti-COL4A2 antibody (ab125208)Anti-COL4A2 antibody (ab125208) at 0.25 µg/ml + Human placenta tissue lysates at 30 µg
Secondary
Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 168 kDaAfter electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT.
The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling COL4A2 with ab125208 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125208 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling COL4A2 with ab125208 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125208 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody (ab125208)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling COL4A2 with ab125208 at 2 ug/ml. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C.
The section was blocked with 10% goat serum then incubated with ab125208 overnight at 4°C.
The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
数据表及文件
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Datasheet download
文献 (1)
ab125208 被引用在 1 文献中.
- Pei J et al. Type IV collagen-derived angiogenesis inhibitor: canstatin low expressing in brain-invasive meningiomas using liquid chromatography-mass spectrometry (LC-MS/MS). J Neurooncol 161:415-423 (2023). PubMed: 36811765
