Anti-COL22A1抗体(ab121846)
Key features and details
- Rabbit polyclonal to COL22A1
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-COL22A1抗体 -
描述
兔多克隆抗体to COL22A1 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human COL22A1 aa 150-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8NFW1 -
阳性对照
- IHC-P: Human stomach and small intestine tissues; ICC: Human cell line U-2 OS.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121846于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P | (3) |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Acts as a cell adhesion ligand for skin epithelial cells and fibroblasts. -
组织特异性
Restrictive expression is observed at tissue junctions such as the myotendinous junction in skeletal and heart muscle, the articular cartilage-synovial fluid junction, or the border between the anagen hair follicle and the dermis in the skin. It is deposited in the basement membrane zone of the myotendinous junction and the hair follicle and associated with the extrafibrillar matrix in cartilage. -
序列相似性
Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Contains 16 collagen-like domains.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 1 VWFA domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 169044 Human
- Omim: 610026 Human
- SwissProt: Q8NFW1 Human
- Unigene: 117169 Human
-
别名
- 2310067L16Rik antibody
- C80743 antibody
- COL22A1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL22A1 antibody (ab121846)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry - Anti-COL22A1 antibody (ab121846)Immunocytochemistry analysis of human cell line U-2 OS cells labelling COL22A1 with ab121846 at 2 µg/mL
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL22A1 antibody (ab121846)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL22A1 antibody (ab121846)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL22A1 antibody (ab121846)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling COL22A1 with ab121846 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab121846 被引用在 6 文献中.
- Morin A et al. Dystrophin myonuclear domain restoration governs treatment efficacy in dystrophic muscle. Proc Natl Acad Sci U S A 120:e2206324120 (2023). PubMed: 36595689
- Huang ML & Luo WL Engrailed homeobox 1 transcriptional regulation of COL22A1 inhibits nasopharyngeal carcinoma cell senescence through the G1/S phase arrest. J Cell Mol Med 26:5473-5485 (2022). PubMed: 36196630
- Zhang Y et al. Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nat Commun 13:2447 (2022). PubMed: 35508470
- Pan R et al. A novel immune cell signature for predicting osteosarcoma prognosis and guiding therapy. Front Immunol 13:1017120 (2022). PubMed: 36189307
- Ishibashi K et al. Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments. Commun Biol 5:1233 (2022). PubMed: 36371589
- Scott RW et al. Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration. Cell Stem Cell 25:797-813.e9 (2019). PubMed: 31809738