AB54532

Anti-Cofilin抗体[1A1]

Name: Cofilin抗体[1A1] - Loading Control (ab54532)| Abcam中文官网
Brand: Abcam Limited
SKU: AB54532
Price: 3959 CNY
Availability: InStock
Rating: 4 (6 reviews)

Anti-Cofilin antibody [1A1] - Loading Control

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(6 Reviews)

(37 Publications)

Mouse Monoclonal Cofilin-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 37 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CFL1.

查看别名

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

6 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cofilin with ab54532 at 1.5 μg/ml in immunohistochemical analysis.

This image was generated using the ascites version of the product.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab54532 at 10 μg/ml in ICC/IF.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 25 µg

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg/mL

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg/mL

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Predicted band size: 18 kDa

false

CiteAb

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western Blotting using Anti-Cofilin antibody [1A1], ab54532. Publication image from Gutierrez-Hartmann, A. et al., 2011, Mol Cancer, 21682918. Legend direct from paper.

Expression and phosphorylation status of GFP-LIMK1 fusion proteins and cofilin. Western blot analysis of whole cell lysates from MDA-MB-231 stable transductants probed for (A) LIMK1 using rat monoclonal antibody and for tubulin using anti-tubulin antibody; (B) probed for phosphoryated LIMK1 at T508 and tubulin (endogenous pLIMK1 is not detectable by Western blot with this antibody); (C) probed for total cofilin and tubulin; and, (D) probed for phosphoryated cofilin at Ser3 and tubulin.

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1A1

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human CFL1.

P23528

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40563335

2025

CSE-Induced ER-Mitochondria Crosstalk Promotes Oxidative Stress and Impairs Bronchial Contractile Response.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Rodríguez-Pérez,Rosa Andreu-Martínez,Leila Pérez-Sánchez,Ana Hernández-García,Cecilia Muñoz-Calleja,Ángel Cogolludo,María J Calzada

Cell death & disease 15:264 PubMed38615035

2024

α-Synuclein triggers cofilin pathology and dendritic spine impairment via a PrP-CCR5 dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Marina I Oliveira da Silva,Miguel Santejo,Isaac W Babcock,Ana Magalhães,Laurie S Minamide,Seok-Joon Won,Erika Castillo,Ellen Gerhardt,Christiane Fahlbusch,Raymond A Swanson,Tiago F Outeiro,Ricardo Taipa,Michael Ruff,James R Bamburg,Márcia A Liz

Genes, brain, and behavior 22:e12863 PubMed37575018

2023

Impaired erythropoietin-producing hepatocellular B receptors signaling in the prefrontal cortex and hippocampus following maternal immune activation in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqian Shao,Yaqi Cai,Tengfei Chen,Keke Hao,Binbin Luo,Xiujuan Wang,Weiyun Guo,Xi Su,Luxian Lv,Yongfeng Yang,Wenqiang Li

Frontiers in molecular neuroscience 15:1013182 PubMed36277486

2022

Plasticity of the hypocretinergic/orexinergic system after a chronic treatment with suvorexant in rats. Role of the hypocretinergic/orexinergic receptor 1 as an autoreceptor.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Carrera-Cañas,Isabel de Andrés,Marta Callejo,Miguel Garzón

The EMBO journal 41:e110192 PubMed36149731

2022

Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Mazille,Katarzyna Buczak,Peter Scheiffele,Oriane Mauger

Theranostics 12:5034-5050 PubMed35836807

2022

SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Rongjun Zou,Wanting Shi,Na Zhou,Shaoxian Chen,Hao Zhou,Xinxin Chen,Yueheng Wu

Frontiers in medicine 9:861371 PubMed35492354

2022

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong Zhuo

International journal of molecular sciences 23: PubMed35269714

2022

Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Krishnatej Nishtala,Trailokyanath Panigrahi,Rohit Shetty,Dhanananajay Kumar,Pooja Khamar,Rajiv R Mohan,Vrushali Deshpande,Arkasubhra Ghosh

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:2190-2204 PubMed35135857

2022

Inositol Polyphosphate-5-Phosphatase K () Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Sierra D Kauer,Kathryn L Fink,Elizabeth H F Li,Brian P Evans,Noa Golan,William B J Cafferty

The Journal of cell biology 221: PubMed35024764

2022

Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adam N Keen,Luke A Payne,Vedanta Mehta,Alistair Rice,Lisa J Simpson,Kar Lai Pang,Armando Del Rio Hernandez,John S Reader,Ellie Tzima

View all publications

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Anti-Cofilin抗体[1A1]

Anti-Cofilin antibody [1A1] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原