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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-Cofilin抗体[1A1] (ab54532)

  • Datasheet
Reviews (6)Q&A (11)References (28)

Product price, shipping and contact information

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Western blot - Anti-Cofilin antibody [1A1] (ab54532)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] (ab54532)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] (ab54532)
  • Western blot - Anti-Cofilin antibody [1A1] (ab54532)
  • Western blot - Anti-Cofilin antibody [1A1] (ab54532)

Key features and details

  • Mouse monoclonal [1A1] to Cofilin
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-Cofilin抗体[1A1]
    参阅全部 Cofilin-1 一抗
  • 描述

    小鼠单克隆抗体[1A1] to Cofilin
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Cow, Pig, Cynomolgus monkey
  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Human Cofilin. AAH11005
    Sequence:

    MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILE EGKEILVGDVGQTVDDPYATFAKMLPDKDCRYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEGVKDRCTLA EKLGGSAVISLEGKPL


    Database link: P23528
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: HeLa, PC-12 and NIH/3T3 cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    1A1
  • 同种型

    IgG2a
  • 轻链类型

    kappa
  • 研究领域

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Cardiovascular
    • Vasculature
    • Smooth muscle cell (SMC)
    • Cytoskeleton
    • Isotype/Loading Controls
    • Loading Controls
    • Cofilin

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human Cofilin protein (ab62958)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab54532于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (5)
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • 组织特异性

    Widely distributed in various tissues.
  • 序列相似性

    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • 翻译后修饰

    Phosphorylated on Ser-3 in resting cells.
  • 细胞定位

    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Target information above from: UniProt accession P23528 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1072 Human
    • Entrez Gene: 12631 Mouse
    • Entrez Gene: 29271 Rat
    • Omim: 601442 Human
    • SwissProt: P23528 Human
    • SwissProt: P18760 Mouse
    • SwissProt: P45592 Rat
    • Unigene: 170622 Human
    • Unigene: 329655 Mouse
    • Unigene: 11675 Rat
    see all
  • 别名

    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

图片

  • Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Anti-Cofilin antibody [1A1] (ab54532) at 1 µg + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 25 µg

    Predicted band size: 18 kDa



    This image was generated using the ascites version of the product

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] (ab54532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] (ab54532)

    Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cofilin with ab54532 at 1.5 µg/ml in immunohistochemical analysis.

    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] (ab54532)
    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] (ab54532)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab54532 at 10 µg/ml in ICC/IF.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Anti-Cofilin antibody [1A1] (ab54532) at 1 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 18 kDa



    This image was generated using the ascites version of the product.

  • Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Western blot - Anti-Cofilin antibody [1A1] (ab54532)
    Anti-Cofilin antibody [1A1] (ab54532) at 1 µg/ml + PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

    Predicted band size: 18 kDa



    This image was generated using the ascites version of the product.

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (28)

发表研究结果有使用 ab54532?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab54532 被引用在 28 文献中.

  • Keen AN  et al. Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells. J Cell Biol 221:N/A (2022). PubMed: 35024764
  • Kauer SD  et al. Inositol Polyphosphate-5-Phosphatase K (Inpp5k) Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma. J Neurosci 42:2190-2204 (2022). PubMed: 35135857
  • Nishtala K  et al. Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing. Int J Mol Sci 23:N/A (2022). PubMed: 35269714
  • Feng L  et al. Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway. Front Med (Lausanne) 9:861371 (2022). PubMed: 35492354
  • Carrera-Cañas C  et al. Plasticity of the hypocretinergic/orexinergic system after a chronic treatment with suvorexant in rats. Role of the hypocretinergic/orexinergic receptor 1 as an autoreceptor. Front Mol Neurosci 15:1013182 (2022). PubMed: 36277486
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 17 Abreviews or Q&A

Western blot abreview for Anti-Cofilin antibody [1A1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (progenitor T cells)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
60 µg
Specification
progenitor T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 03 2021

Western blot abreview for Anti-Cofilin antibody [1A1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
55 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 01 2021

Western blot abreview for Anti-Cofilin antibody [1A1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
67 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 13 2021

Western blot abreview for Anti-Cofilin antibody [1A1]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (T-ALL cell line)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
100 µg
Specification
T-ALL cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 10 2021

Immunoprecipitation abreview for Anti-Cofilin antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12, rat pheochromacytoma)
Total protein in input
1000 µg
Specification
PC12, rat pheochromacytoma
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 31 2012

Question

I would like to avail of the offer to test ab54532 in IP as part of the testing discount offer.

Regards,

Read More

Abcam community

Verified customer

Asked on Sep 05 2012

Answer

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab54532 in IP.

Please place your order for ab54532 with the following discount code. This first order will be invoiced at full price:

DISCOUNT CODE: 100%ABR-EB34E
Expiration date: 5th Jan 2013

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer once you have tested the antibody, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Sep 05 2012

Question

I would be interested in testing ab54532 for IP if I could take part in the testing discount program. Could I ask, can I use the discount vouchers collected from abpoints to buy ab54532 and still take part in the discount program? Or do I need to pay full price for the antibody to be eligible?

Thanks,

Read More

Abcam community

Verified customer

Asked on Sep 05 2012

Answer

Thank you very much for your reply. I am pleased to hear you are interested in the testing discount offer.

I can offer a discount off a future purchase if you buy ab54532 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab54532 in IP. I will then send a discount code. This code must be issued before purchasing ab54532 so please wait for my reply before ordering.

2. Purchase ab54532 either by phone, fax, or online (https://www.abcam.com).

I am sorry you will not be able to use the Abpoints for this purchase if you are going to use the testing discount code.

3. Test it in IP.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab 54532 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Sep 05 2012

Question

To Whom It May Concern,

The datasheet information on the Cofilin antibody (ab54532) only mentions use in western blotting, IHC and IF. Is there any information on the use of this antibody in immunoprecipitation?
What are the chances, based on the epitope used that the antibody will work in this application?
I appreciate any information you can give me.

Yours sincerely,

Read More

Abcam community

Verified customer

Asked on Sep 04 2012

Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab54532 has never been tested in IP. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated as soon as any new information is brought to our attention.

If you would like to test ab54532Cofilin antibodyin IP, please contact me prior to the purchase by replying to this message as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More

Abcam Scientific Support

回复于 Sep 04 2012

Question

Hi,
I am facing problem with one of your product Ab54532, all detail
is given below,
1) Abcam product code: ab54532
2) Abcam order reference number or product batch number:

3) Description of the problem:
We used this Product for Immunoprecipitation, to capture the
antigen in the cell culture supernatant then analyzed by western blot
by using ab42824 but there was no different between control(only
treated with Magnetic beads) and samples (treated magnetic beads and
ab54532). The Product ab42824 is working very well for normal western
blot. Then we tried Ab54532 for normal western blot by loading 50ug of
protein extract, found that not working from even from 2000-250
dilution. But product Ab42824 is working at 3500 dilution for normal
western blot with 2ug of samples.
4) Sample preparation:
Type of sample (whole cell lysates, fraction, recombinant protein…):
Whole cell lysates
Lysis buffer: Urea lysis buffer
Protease inhibitors: PIC EDTA free
Phosphatase inhibitors
Reducing agent : SDS Sample Buffer
Boiling for ≥5 min? yes
Protein loaded ug/lane or cells/lane: 50ug
Positive control
Negative control
5) Percentage of gel: 12% Acrylamide
Type of membrane: Nitrocellulose
Protein transfer verified: Staining of residual Gel
Blocking agent and concentration: 5% BSA or Milk Powder
Blocking time : 1hr
Blocking temperature: Room Temperature
6) Primary antibody (If more than one was used, describe in
“additional notes”) :
Concentration or dilution: 2000-250
Diluent buffer : 5%BSA or Milk Powder
Incubation time: over night
Incubation temperature: 4oC
7) Secondary antibody:
Species: Rabbit
Reacts against: Mouse
Concentration or dilution: 3000-1000
Diluent buffer: 5% BSA or Milk Powder
Incubation time: 1-2 hrs
Incubation temperature: Room Temperature
Fluorochrome or enzyme conjugate: HRP
8) Washing after primary and secondary antibodies:
Buffer: TBS-tween
Number of washes: after primary (3x5) and after Secondary (3x15)
9) Detection method: By using ECL reagent.
10) How many times have you run this staining? More than 4 times
Do you obtain the same results every time? YES
What steps have you altered to try and optimize the use of this antibody?
By Optimizing blocking steps by BSA and milk powder, amount of samples
from 20-50ug, Primary Antibody dilutions from 2000-250, Secondary
antibody dilution from 3000-1000
Document attachment: Attaching images of your blot is strongly
recommended and can greatly speed up our investigation of your problem.
 Nothing on the images…..
Regards,

Read More

Abcam community

Verified customer

Asked on Aug 09 2012

Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from ab54532. The details you have kindly provided will provide us with vital information for our monitoring of product quality
I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial.
Pleas note that ab54532 is not tested for IP and we do not know if it is suitable.
I apologise for the inconvenience and am pleased to offer you a free of charge replacement (also possible with an alternative antibody; unfortunately, we do not have an anti-Cofilin antibody that is tested for IP) or credit note in compensation.
Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Aug 09 2012

Question

phone call; do you know the epitope?

Read More

Abcam community

Verified customer

Asked on Apr 13 2012

Answer

Merci pour votre appel et votre patience.
Le laboratoire vient de me confirmer que ils n'ont pas fait un "epitope mapping" sur cet anticorps, et donc que l'épitope de cet anticorps n'est pas connue. Effectivement, l'immunogène était toute la protéine Cofilin avec un tag, et l'épitope peux donc être théoriquement n'importe ou sur la protéine.
Je suis désolée de ne pas pouvoir vous aider plus en cette occasion. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Abcam Scientific Support

回复于 Apr 13 2012

1-10 of 17 Abreviews or Q&A

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研究类别(A-Z)
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