Anti-CNBP抗体(ab48027)
Key features and details
- Goat polyclonal to CNBP
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CNBP抗体
参阅全部 CNBP 一抗 -
描述
山羊多克隆抗体to CNBP -
宿主
Goat -
特异性
No crossreaction expected with CCHC domain containing 13. -
经测试应用
适用于: Flow Cyt, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus monkey, Macaque monkey -
免疫原
Synthetic peptide:
GESGHLARECTIE
, corresponding to C terminal amino acids 162-174 of Human CNBP. -
阳性对照
- Flow Cyt: MCF7 cells ICC/IF: Neuro-2a and MCF2 cells WB: Neuro2a, MCF7, MOLT-4 and Daudi cell lysate; Human skeletal muscle tissue lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48027于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB | (3) |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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Flow Cyt
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. -
组织特异性
Present in all tissues examined. -
疾病相关
Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. -
序列相似性
Contains 7 CCHC-type zinc fingers. -
细胞定位
Cytoplasm. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 395731 Chicken
- Entrez Gene: 504831 Cow
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 399294 Xenopus laevis
- Omim: 116955 Human
- SwissProt: O42395 Chicken
- SwissProt: Q3T0Q6 Cow
see all -
别名
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- Cellular nucleic acid-binding protein antibody
see all
图片
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All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : MOLT-4 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Neuro2a cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 19 kDaLysate in RIPA buffer. Detected by chemiluminescence.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Anti-CNBP antibody (ab48027) at 1 µg/ml + Human Skeletal Muscle lysate at 35 µg
Predicted band size: 19 kDaLysate in RIPA buffer. Detected by chemiluminescence.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed Neuro-2a cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 19 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 6 minutes
数据表及文件
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Datasheet download
文献 (4)
ab48027 被引用在 4 文献中.
- Wang Y et al. Circular RNA circDVL1 inhibits clear cell renal cell carcinoma progression through the miR-412-3p/PCDH7 axis. Int J Biol Sci 18:1491-1507 (2022). PubMed: 35280687
- Coni S et al. Translational control of polyamine metabolism by CNBP is required for Drosophila locomotor function. Elife 10:N/A (2021). PubMed: 34517941
- Yang F et al. Circ-HuR suppresses HuR expression and gastric cancer progression by inhibiting CNBP transactivation. Mol Cancer 18:158 (2019). PubMed: 31718709
- Antonucci L et al. CNBP regulates wing development in Drosophila melanogaster by promoting IRES-dependent translation of dMyc. Cell Cycle 13:434-9 (2014). PubMed: 24275942