Anti-CNBP抗体(ab272676)
Key features and details
- Rabbit polyclonal to CNBP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CNBP抗体
参阅全部 CNBP 一抗 -
描述
兔多克隆抗体to CNBP -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Orangutan
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免疫原
Recombinant fragment corresponding to Human CNBP aa 1-100.
Database link: P62633 -
阳性对照
- WB: RT4, U-251 MG cells, Human liver and tonsil. IF: SH-SY5Y cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272676于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression. -
组织特异性
Present in all tissues examined. -
疾病相关
Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene. -
序列相似性
Contains 7 CCHC-type zinc fingers. -
细胞定位
Cytoplasm. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 64530 Rat
- Omim: 116955 Human
- SwissProt: P62633 Human
- SwissProt: P53996 Mouse
- SwissProt: P62634 Rat
- Unigene: 518249 Human
see all -
别名
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- Cellular nucleic acid-binding protein antibody
see all
图片
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Western blot - Anti-CNBP antibody (ab272676)All lanes : Anti-CNBP antibody (ab272676) at 0.4 µg/ml
Lane 1 : RT4 cells (Human urinary bladder cancer cell line)
Lane 2 : U-251 MG cells (Human brain glioma cell line)
Lane 3 : Human plasma
Lane 4 : Human liver tissue
Lane 5 : Human tonsil tissue -
Immunocytochemistry/ Immunofluorescence - Anti-CNBP antibody (ab272676)PFA fixed, Triton X-100 permeablized SH-SY5Y cells (human neuroblastoma cell line from bone marrow) labeling CNBP (green) using ab272676 at 2 ug/ml in ICC/IF analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab272676 被引用在 1 文献中.
- Fan C et al. CircFMN2 Boosts Sorafenib Resistance in Hepatocellular Carcinoma Cells via Upregulating CNBP by Restraining Ubiquitination. J Oncol 2022:2674163 (2022). PubMed: 35909906