AB229127

重组Anti-Clusterin beta chain抗体[EPR17538-101] - BSA and Azide free

Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Clusterin antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat samples. Cited in 1 publication.

查看别名

Apoj, Msgp-2, Clu, Clusterin, Apolipoprotein J, Clustrin, Sulfated glycoprotein 2, Apo-J, SGP-2

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in stromal cells of mouse spleen (PMID : 24865838) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184099).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

This data was developed using ab184099, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Adrenal gland tissue from wild-type C57BL/6J mice (B) Adrenal gland tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Adrenal gland tissue from wild-type C57BL/6J mice and no staining on (B) Adrenal gland tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument.The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID : T014419).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

This data was developed using ab184099, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6J mice (B) Spleen tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6J mice and no staining on (B) Spleen tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID : T014419).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

This data was developed using ab184099, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) Testis tissue from wild-type C57BL/6J mice (B) Testis tissue from Clusterin knockout mice staining with ab184099 at 1/2000 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Testis tissue from wild-type C57BL/6J mice and no staining on (B) Testis tissue from Clusterin knockout mice. The section was incubated with ab184099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID : T014419).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Clusterin with ab184099 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Stronger cytoplasmic staining in the medulla than the cortex of rat adrenal gland (PMID : 16436671) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab184099).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

This data was developed using ab184099, the same antibody clone in a different buffer formulation. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and Clusterin-KO homozygous mice (Strain ID : T014419).

All lanes:

Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (<a href='/products/primary-antibodies/clusterin-beta-chain-antibody-epr17538-101-ab184099'>ab184099</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse adrenal gland tissue lysate (male)

Lane 2:

Clusterin knockout adrenal gland tissue lysate (male)

Lane 3:

Wild-type mouse spleen tissue lysate (male)

Lane 4:

Clusterin knockout spleen tissue lysate (male)

Lane 5:

Wild-type mouse testis tissue lysate (male)

Lane 6:

Clusterin knockout testis tissue lysate (male)

Lane 7:

Wild-type mouse adrenal gland tissue lysate (female)

Lane 8:

Clusterin knockout adrenal gland tissue lysate (female)

Lane 9:

Wild-type mouse spleen tissue lysate (female)

Lane 10:

Clusterin knockout spleen tissue lysate (female)

false

Supplier Data

Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] - BSA and Azide free (AB229127)

Blocking and dilution buffer : 5% NFDM/TBST .

Exposure times.

Lanes 1, 2 & 3 : 3 minutes.

Lane 4 : 53 seconds.

Lanes 5 & 6 : 100 seconds.

The molecular masses observed are consistent with what has been described in the literature (PMID : 8373947).

The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument

All lanes:

Western blot - Anti-Clusterin beta chain antibody [EPR17538-101] (<a href='/products/primary-antibodies/clusterin-beta-chain-antibody-epr17538-101-ab184099'>ab184099</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

Mouse adrenal gland tissue lysate at 20 µg

Lane 3:

Rat adrenal gland tissue lysate at 20 µg

Lane 4:

Rat lung tissue lysate at 20 µg

Lane 5:

Mouse stomach tissue lysate at 20 µg

Lane 6:

Rat stomach tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 33 kDa,70 kDa

true

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR17538-101

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab229127 is the carrier-free version of ab184099.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Clusterin beta chain also known as Apolipoprotein J is part of the clusterin protein complex that goes through heterodimerization to form a mature glycoprotein composed of alpha and beta chains. The estimated mass of the clusterin beta chain subunit is around 35-40 kDa. This protein is present in many tissues throughout the body with higher expression noted in the brain testes and kidneys as well as in blood plasma. It functions in molecular chaperoning and stabilizes numerous proteins and cells under stressful conditions.

Biological function summary

Clusterin beta chain engages in diverse functions related to cell apoptosis cell signaling and lipid transport. It is part of a secreted glycoprotein complex that includes an alpha chain. In the extracellular space it plays a significant role in inhibiting programmed cell death while also engaging in the regulation of immune responses. Its molecular chaperone capability safeguards cells from aggregation-prone stressors.

Pathways

Clusterin beta chain interacts with pathways associated with neuroprotection and immune regulation. It significantly participates in the apoptosis signaling pathway where it relates to other proteins such as Bax and Bcl-2 family members. Additionally its presence in the complement cascade relates it to complement component proteins such as C1q and C3 indicating its involvement in immune responses.

Clusterin beta chain relates strongly to neurodegenerative diseases and some cancers. In Alzheimer's disease its interaction with amyloid-beta highlights its potential impact in amyloid plaque formation and clearance. It associates with amyloid precursor proteins in the disease mechanism. Furthermore in various cancer progressions its altered expression links to tumor suppression and metastasis. Proteins like p53 and Hsp70 display interactions with clusterin beta chain within these contexts influencing tumor cell survival and spread.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins (By similarity). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed : 14741101). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity (By similarity). Following stress, promotes apoptosis (PubMed : 12551933). Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. Following ER stress, suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. When secreted, does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (By similarity). When secreted, acts as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (PubMed : 11865066).

See full target information Clu

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