重组Anti-CLSTN1抗体[EPR2963] (ab134130)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2963] to CLSTN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CLSTN1抗体[EPR2963]
参阅全部 CLSTN1 一抗 -
描述
兔单克隆抗体[EPR2963] to CLSTN1 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 91% homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human CLSTN1 (C terminal). The exact sequence is proprietary.
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阳性对照
- Human fetal brain lysate; 293T cell lysate; U87 MG lysate; SH SY5Y lysate and Neuro 2a lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2963 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134130于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 110 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals. -
组织特异性
Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level). -
序列相似性
Contains 2 cadherin domains. -
结构域
The cytoplasmic domain is involved in interaction with APBA2, as well as the binding of synaptic Ca(2+). -
翻译后修饰
Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection. Cell junction > synapse > postsynaptic cell membrane. Nucleus. Neurite tips. Localized in the postsynaptic membrane of both excitatory and inhibitory synapses (By similarity). The AlcICD fragment is translocated to the nucleus upon interaction with APBB1. - Information by UniProt
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数据库链接
- Entrez Gene: 22883 Human
- Entrez Gene: 65945 Mouse
- Omim: 611321 Human
- SwissProt: O94985 Human
- SwissProt: Q9EPL2 Mouse
- Unigene: 29665 Human
- Unigene: 38993 Mouse
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别名
- Alc alpha antibody
- Alc-alpha antibody
- Alcadein alpha 1 antibody
see all
图片
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Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : 293T cell lysate
Lane 3 : U87 MG lysate
Lane 4 : SH SY5Y lysate
Lane 5 : Neuro 2a lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti mouse at 1/2000 dilution
Predicted band size: 110 kDa -
Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/200000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Extra band at: 90kDa We are unsure as to the identity of this extra band.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab134130 被引用在 3 文献中.
- Ou MY et al. The CTNNBIP1-CLSTN1 fusion transcript regulates human neocortical development. Cell Rep 35:109290 (2021). PubMed: 34192541
- Lleó A et al. Changes in Synaptic Proteins Precede Neurodegeneration Markers in Preclinical Alzheimer's Disease Cerebrospinal Fluid. Mol Cell Proteomics 18:546-560 (2019). PubMed: 30606734
- Yu L et al. The Circular RNA Cdr1as Act as an Oncogene in Hepatocellular Carcinoma through Targeting miR-7 Expression. PLoS One 11:e0158347 (2016). PubMed: 27391479