重组Anti-CLPTM1抗体[EPR8800] (ab133756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8800] to CLPTM1
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CLPTM1抗体[EPR8800]
参阅全部 CLPTM1 一抗 -
描述
兔单克隆抗体[EPR8800] to CLPTM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human CLPTM1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O96005 -
阳性对照
- WB: A549, HAP1, HeLa, 293T, U87 MG, HepG2 and Jurkat cell lysate, IHC: Papillary adenocarcinoma of Human thyroid gland tissue. ICC/IF: HeLa cells Flow Cyt: A549 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 3.45 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8800 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133756于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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靶标
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功能
May play a role in T-cell development. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the CLPTM1 family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1209 Human
- Omim: 604783 Human
- SwissProt: O96005 Human
- Unigene: 444441 Human
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别名
- Cleft lip and palate associated transmembrane protein 1 antibody
- Cleft lip and palate transmembrane protein 1 antibody
- CLPT1_HUMAN antibody
see all
图片
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All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CLPTM1 knockout HAP1 whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76 kDa
Observed band size: 76 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133756 observed at 76 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133756 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in CLPTM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CLPTM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab133756 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLPTM1 antibody [EPR8800] (ab133756)
Immunohistochemical analysis of paraffin embedded papillary adenocarcinoma of Human thyroid gland tissue labelling CLPTM1 with ab133756 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling CLPTM1 with purified ab133756 at 1/200 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunofluorescent staining of HeLa cells labelling CLPTM1 with ab133756 at 1/250.
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All lanes : Anti-CLPTM1 antibody [EPR8800] (ab133756) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87 MG cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 76 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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