重组Anti-Clostridium difficile Toxin A抗体[EPR23359-15] (ab272720)
![Western blot - Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720)](https://www.abcam.cn/ps/products/272/ab272720/Images/ab272720-393134-AntiClostridium-difficile-Toxin-A-antibody-EPR2335915westernblot.jpg "Western blot - Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23359-15] to Clostridium difficile Toxin A
- Suitable for: WB
- Reacts with: Clostridium difficile
Related conjugates and formulations
概述
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产品名称
Anti-Clostridium difficile Toxin A抗体[EPR23359-15]
参阅全部 Clostridium difficile Toxin A 一抗 -
描述
兔单克隆抗体[EPR23359-15] to Clostridium difficile Toxin A -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Clostridium difficile -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Native Clostridium difficile Toxin A protein.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23359-15 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272720于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 308 kDa).
(0.512 μg/ml). |
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 308 kDa (predicted molecular weight: 308 kDa). (0.512 μg/ml). |
靶标
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相关性
Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine. -
细胞定位
Secreted -
数据库链接
- SwissProt: P16154 Clostridium difficile
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别名
- tcdA antibody
- toxA antibody
图片
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Western blot - Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720)All lanes : Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720) at 1/1000 dilution
Lane 1 : Native Clostridium difficile Toxin A protein (ab123999) 10ng
Lane 2 : Native Clostridium difficile Toxin B protein (ab124001) 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 308 kDa
Observed band size: 308 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Both native proteins were from Abcam.
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![Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720)](https://www.abcam.cn/ps/products/272/ab272720/Images/ab272720-2-benefits-of-recombinant-antibodies.png)
Anti-Clostridium difficile Toxin A antibody [EPR23359-15] (ab272720)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab272720 尚未被引用在任何文献中。