Key features and details
- Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Clostridium botulinum
- Isotype: IgG
产品名称Anti-Clostridium botulinum E Toxoid抗体[209F2]
描述小鼠单克隆抗体[209F2] to Clostridium botulinum E Toxoid
特异性BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16)
经测试应用适用于: ICC/IF, Inhibition Assaymore details
种属反应性与反应: Clostridium botulinum
Synthetic peptide analogue of the amino terminal region of BoTox-E.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Constituent: Tissue culture supernatant
Concentration information loading...
纯度Tissue culture supernatant
纯化说明Sterile filtered culture supernatant.
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This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity.
- SwissProt: Q00496 Clostridium botulinum
- BoNT/E antibody
- Bontoxilysin e antibody
- Botulinum neurotoxin type e non toxic component antibody
ab40787 被引用在 1 文献中.
- Shu Y et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). PubMed: 18171919