Anti-Clostridium botulinum E Toxoid抗体[209F2] (ab40787)
Key features and details
- Mouse monoclonal [209F2] to Clostridium botulinum E Toxoid
- Suitable for: ICC/IF, Inhibition Assay
- Reacts with: Clostridium botulinum
- Isotype: IgG
概述
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产品名称
Anti-Clostridium botulinum E Toxoid抗体[209F2] -
描述
小鼠单克隆抗体[209F2] to Clostridium botulinum E Toxoid -
宿主
Mouse -
特异性
BoTox-E (2-17) 100%; BoTox-E 100% Does not cross react with BoTox-A, BoTox-B, BoTox-C or BoTox-F (1-16) -
经测试应用
适用于: ICC/IF, Inhibition Assaymore details -
种属反应性
与反应: Clostridium botulinum -
免疫原
Synthetic peptide analogue of the amino terminal region of BoTox-E.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituent: Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Sterile filtered culture supernatant. -
克隆
单克隆 -
克隆编号
209F2 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Inhibition Assay |
| 说明 |
|---|
应用说明
IF: 1/50.
This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This product has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure.
Inhib: Use at an assay dependent dilution.
This product has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.
With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
Clostridium botulinum E Toxoid acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity. -
细胞定位
Secreted -
数据库链接
- SwissProt: Q00496 Clostridium botulinum
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别名
- BoNT/E antibody
- Bontoxilysin e antibody
- Botulinum neurotoxin type e non toxic component antibody
see all
数据表及文件
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Datasheet download
文献 (1)
ab40787 被引用在 1 文献中.
- Shu Y et al. Phosphorylation of SNAP-25 at Ser187 mediates enhancement of exocytosis by a phorbol ester in INS-1 cells. J Neurosci 28:21-30 (2008). PubMed: 18171919
