Anti-CLMN抗体(ab121373)
Key features and details
- Rabbit polyclonal to CLMN
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CLMN抗体 -
描述
兔多克隆抗体to CLMN -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen, corresponding to amino acids 446-553 of Human CLMN.
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阳性对照
- Human liver lysate; Human kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121373于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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组织特异性
Widely expressed at intermediate level. -
序列相似性
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 79789 Human
- Omim: 611121 Human
- SwissProt: Q96JQ2 Human
- Unigene: 301478 Human
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别名
- 9330188N17Rik antibody
- AI428889 antibody
- AI788815 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLMN antibody (ab121373)
Immunohistochemical analysis of human testis tissue labelled for CLMN using ab121373 at 1/200 diltuion. Positive cytoplasmic staining observed in siminiferous duct cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLMN antibody (ab121373)Immunohistochemical analysis of human prostate tissue labelled for CLMN using ab121373 at 1/200 diltuion. Positive membranous staining observed in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLMN antibody (ab121373)Immunohistochemical analysis of human colon tissue labelled for CLMN using ab121373 at 1/200 diltuion. Positive membranous and cytoplasmic staining observed in glandular cells.
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Immunocytochemistry/ Immunofluorescence - Anti-CLMN antibody (ab121373)Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121373 1-4 µg/ml. Cells treated with PFA/Triton X-100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab121373 被引用在 1 文献中.
- Fukuzawa R et al. The developmental programme for genesis of the entire kidney is recapitulated in Wilms tumour. PLoS One 12:e0186333 (2017). Mouse . PubMed: 29040332