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AB312846

重组Anti-CLIPR-59抗体[EPR28002-60]

Anti-CLIPR-59 antibody [EPR28002-60]

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Rabbit Recombinant Monoclonal CLIPR-59 antibody. Suitable for WB, IP and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

查看别名

CLIPR59, CLIP3, CAP-Gly domain-containing linker protein 3, Cytoplasmic linker protein 170-related 59 kDa protein, CLIP-170-related 59 kDa protein, CLIPR-59

6 Images
Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • IP

Supplier Data

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

CLIPR-59 was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab312846 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312846 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Rat brain tissue lysate 10 ug Lane 2 : Rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab312846 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/30 dilution

All lanes:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • IP

Supplier Data

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

CLIPR-59 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab312846 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab312846 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate Lane 2 : Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab312846 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/30 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • WB

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : heart (PMID : 19139280), liver (PMID : 19139280). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 60 kDa

false

Exposure time: 92s

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • WB

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (PMID : 19139280). In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Mouse spinal cord tissue lysate at 50 µg

Lane 2:

Mouse brain tissue lysate at 50 µg

Lane 3:

Mouse liver tissue lysate at 50 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 60 kDa

false

Exposure time: 180s

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • WB

Supplier Data

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : heart (PMID : 19139280), liver (PMID : 19139280). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

Rat spinal cord tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 60 kDa

false

Exposure time: 92s

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)
  • WB

Lab

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (AB312846)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This antibody doesn't cross-react with human CLIP4, CLIP2 and CLIP1.

All lanes:

Western blot - Anti-CLIPR-59 antibody [EPR28002-60] (ab312846) at 1/1000 dilution

Lane 1:

GST-tagged human CLIP3/CLIPR-59 recombinant full-length protein at 10 ng

Lane 2:

GST-tagged human CLIP4 recombinant full-length protein at 10 ng

Lane 3:

GST-tagged human CLIP2 recombinant full-length protein at 10 ng

Lane 4:

DDDDK-tagged human CLIP1/CLIP170 HEK293T cell transient overexpression lysate at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 86 kDa

false

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR28002-60

亚型

IgG

不含载体蛋白

No

反应种属

Human, Mouse, Rat

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody doesn't cross-react with human CLIP4, CLIP2 and CLIP1

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>(0.485ug/ml)</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>(0.485ug/ml)</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>(0.485ug/ml)</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>(0.485ug/ml)</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes.
See full target information CLIP3
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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关联产品

Select an associated product type
Alternative Version
Primary Antibodies

AB312847

Anti-CLIPR-59 antibody [EPR28002-60] - BSA and Azide free

primary-antibodies

clipr-59-antibody-epr28002-60-bsa-and-azide-free-ab312847

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